Product nameAnti-TRPC3 antibody - C-terminal
See all TRPC3 primary antibodies
DescriptionRabbit polyclonal to TRPC3 - C-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human TRPC3 (C terminal). 14 amino acids. NP_003296.1.
Database link: Q13507-3
- Human brain tissue lysate, Human skeletal muscle tissue and mouse heart cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab188802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 97 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
RelevanceTRPC channels form the subfamily of channels in human most closely related to drosophila TRP channels. Structurally, this family shares a number of similar characteristics. At the proximal C-terminus of this sub-family is a TRP box motif containing the invariant EWKFAR sequence and between 3 and 4 ankyrin repeats near the N-terminus. These channels are non-selectively permeable to cations, with a selectivity of calcium over sodium variable among the different members. Many of TRPC channel subunits are able to coassemble.
Cellular localizationCell Membrane; multi-pass membrane protein
- Htrp 3 antibody
- Htrp3 antibody
- MGC124333 antibody
Immunofluorescent analysis of mouse heart cells, labeling TRPC3 with ab188802 at 20 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle tissue labeling TRPC3 with ab188802 at 5 µg/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Lane 1 : Anti-TRPC3 antibody - C-terminal (ab188802) at 1 µg/ml
Lane 2 : Anti-TRPC3 antibody - C-terminal (ab188802) at 2 µg/ml
All lanes : Human brain tissue lysate
Predicted band size: 97 kDa
This product has been referenced in:
- Chen Y et al. HERV-W env regulates calcium influx via activating TRPC3 channel together with depressing DISC1 in human neuroblastoma cells. J Neurovirol N/A:N/A (2018). Read more (PubMed: 30397826) »
- Aravamudan B et al. Mechanisms of BDNF regulation in asthmatic airway smooth muscle. Am J Physiol Lung Cell Mol Physiol 311:L270-9 (2016). Read more (PubMed: 27317689) »