Key features and details
- Mouse monoclonal [3F2.H10.F2] to TRPC6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-TRPC6 antibody [3F2.H10.F2]
See all TRPC6 primary antibodies
DescriptionMouse monoclonal [3F2.H10.F2] to TRPC6
SpecificityCross-reactivity with TRPC6 from other sources has not been determined.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide corresponding to a region near the C terminal of Human TRPC6 (NP_004612).
- Human adrenal (cortex) tissue
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityProtein A purified
Purification notesPurified from concentrated tissue culture supernate by Protein A chromatography.
Our Abpromise guarantee covers the use of ab105845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 106 kDa.|
|IHC-P||Use a concentration of 2.5 µg/ml.|
FunctionThought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
Tissue specificityExpressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
Involvement in diseaseDefects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
Contains 4 ANK repeats.
- Information by UniProt
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ab105845 at 2.5 µg/ml staining TRPC6 in Human adrenal (cortex) tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
The image shows strong staining with minimal background staining. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Western blot analysis of ab105845 with mouse kidney (10ug) at the dilution 1/1000.Secondary antibody (DyLight™ 649 donkey anti-mouse) was used at the dilution 1/20000
ab105845 has not yet been referenced specifically in any publications.