Overview

  • Product name
    Anti-TRPC6 antibody [3F2.H10.F2]
    See all TRPC6 primary antibodies
  • Description
    Mouse monoclonal [3F2.H10.F2] to TRPC6
  • Host species
    Mouse
  • Specificity
    Cross-reactivity with TRPC6 from other sources has not been determined.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region near the C terminal of Human TRPC6 (NP_004612).

  • Positive control
    • Human adrenal (cortex) tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab105845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 106 kDa.
ELISA 1/100000 - 1/50000.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function
    Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
  • Tissue specificity
    Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
  • Involvement in disease
    Defects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities
    Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
    Contains 4 ANK repeats.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • bZ1P14.9 antibody
    • FLJ11098 antibody
    • FLJ14863 antibody
    • FSGS2 antibody
    • MTRP6 antibody
    • Short transient receptor potential channel 6 antibody
    • si:rp71-1p14.9 antibody
    • Transient receptor potential cation channel subfamily C member 6 antibody
    • Transient receptor protein 6 antibody
    • TRP 6 antibody
    • TRP-6 antibody
    • TRP6 antibody
    • TRPC 6 antibody
    • Trpc6 antibody
    • TRPC6_HUMAN antibody
    • TRRP6 antibody
    see all

Images

  • ab105845 at 2.5 µg/ml staining TRPC6 in Human adrenal (cortex) tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
    The image shows strong staining with minimal background staining. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
  • Western blot analysis of ab105845 with mouse kidney (10ug) at the dilution 1/1000.Secondary antibody (DyLight™ 649 donkey anti-mouse) was used at the dilution 1/20000

References

ab105845 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Rabbit Tissue lysate - whole (Ventricle)
Specification
Ventricle
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 07 2015

Answer

Thank you very much for your interest in our anti-TRPC6 antibodies.

While we do offer six products which work in human tissues, to our knowledge, none of these products have been tested in FACS on human cells. Therefore, I can offer a discount off a future purchase if you buy one of these now, test it in FACS and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY OR VALUE OFF OF NEXT ORDER.

The products are:
ab63038: https://www.abcam.com/TRPC6-antibody-mAbcam63038-ab63038.html
ab62461: https://www.abcam.com/TRPC6-antibody-Aminoterminal-end-ab62461.html
ab63000: https://www.abcam.com/TRPC6-antibody-ab63000.html
ab63009: https://www.abcam.com/TRPC6-antibody-ab63009.html
ab105845: https://www.abcam.com/TRPC6-antibody-3F2-H10-F2-ab105845.html
ab106840: https://www.abcam.com/TRPC6-antibody-ab106840.html

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test one of these products. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Test it in FACS.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY or PRODUCT ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for FACS, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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