• Product name
  • Description
    Rabbit polyclonal to TRPC6
  • Host species
  • Specificity
    This antibody does potentially cross-react with TRPC3.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRPC6 (N terminal).
    Database link: Q9Y210
    (Peptide available as ab106953)

  • Positive control
    • WB: K562 cell lysate. IHC-P: Mouse lung tissue. ICC/IF: K562 cells.



Our Abpromise guarantee covers the use of ab62461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).Can be blocked with TRPC6 peptide (ab106953).


  • Function
    Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
  • Tissue specificity
    Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
  • Involvement in disease
    Defects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities
    Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
    Contains 4 ANK repeats.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • bZ1P14.9 antibody
    • FLJ11098 antibody
    • FLJ14863 antibody
    • FSGS2 antibody
    • MTRP6 antibody
    • Short transient receptor potential channel 6 antibody
    • si:rp71-1p14.9 antibody
    • Transient receptor potential cation channel subfamily C member 6 antibody
    • Transient receptor protein 6 antibody
    • TRP 6 antibody
    • TRP-6 antibody
    • TRP6 antibody
    • TRPC 6 antibody
    • Trpc6 antibody
    • TRPC6_HUMAN antibody
    • TRRP6 antibody
    see all


  • K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 (red) using ab62461 (5 µg/ml) in ICC/IF.

  • Paraffin embedded mouse lung tissue stained for TRPC6 with ab62461 (10 µg/ml) in immunohistochemical analysis.

  • Lane 1 : Anti-TRPC6 antibody (ab62461) at 0.5 µg/ml
    Lane 2 : Anti-TRPC6 antibody (ab62461) at 1 µg/ml

    All lanes : K562 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 106 kDa
    Observed band size: 106 kDa

  • K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 using ab62461 (2 µg/ml) in ICC.


This product has been referenced in:
  • Yang X  et al. Role of transient receptor potential channel 6 in the odontogenic differentiation of human dental pulp cells. Exp Ther Med 14:73-78 (2017). Read more (PubMed: 28672895) »
  • Takada H  et al. Mechanosensitive ATP release from hemichannels and Ca²? influx through TRPC6 accelerate wound closure in keratinocytes. J Cell Sci 127:4159-71 (2014). ICC ; Human . Read more (PubMed: 25097230) »

See all 3 Publications for this product

Customer reviews and Q&As

I was not able to obtain the exact sequence of the immunogen, as it is considered proprietary by our source for this antibody. I did learn that the immunogen shares 92% identity with rat TRPC6. There are 13 contiguous identical amino acids out of 14. <...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Mouse Cell (Podocyte)
Yes - 0.1% triton-X

Abcam user community

Verified customer

Submitted Jul 27 2012

Please find a new discount code that you can use to submit your Abreview for ab62461 in IF:

new discount code - 1*00%ABR-TBVX1, exp*

If there is anything else I can help you with, please let me know.

Thank you for contacting us. After consulting with the laboratory regarding ab62461, I have discovered that though the rat and mouse sequence are quite similar, they did test in rat and did not receive a positive signal.

I hope this informatio...

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Thank you very much for your interest in our anti-TRPC6 antibodies.

While we do offer six products which work in human tissues, to our knowledge, none of these products have been tested in FACS on human cells. Therefore, I can offer a discoun...

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Expiration date: ************

I am very pleased to hear you would like to accept our offer and test ab62461 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this...

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