Overview

  • Product name

  • Description

    Rabbit polyclonal to TRPC6
  • Host species

    Rabbit
  • Specificity

    This antibody does potentially cross-react with TRPC3.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRPC6 (N terminal).
    Database link: Q9Y210
    (Peptide available as ab106953)

  • Positive control

    • WB: K562 cell lysate. IHC-P: Mouse lung tissue. ICC/IF: K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).

Target

  • Function

    Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
  • Tissue specificity

    Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
  • Involvement in disease

    Defects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities

    Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
    Contains 4 ANK repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • bZ1P14.9 antibody
    • FLJ11098 antibody
    • FLJ14863 antibody
    • FSGS2 antibody
    • MTRP6 antibody
    • Short transient receptor potential channel 6 antibody
    • si:rp71-1p14.9 antibody
    • Transient receptor potential cation channel subfamily C member 6 antibody
    • Transient receptor protein 6 antibody
    • TRP 6 antibody
    • TRP-6 antibody
    • TRP6 antibody
    • TRPC 6 antibody
    • Trpc6 antibody
    • TRPC6_HUMAN antibody
    • TRRP6 antibody
    see all

Images

  • Lane 1 : Anti-TRPC6 antibody (ab62461) at 0.5 µg/ml
    Lane 2 : Anti-TRPC6 antibody (ab62461) at 1 µg/ml

    All lanes : K562 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 106 kDa
    Observed band size: 106 kDa

  • K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 (red) using ab62461 (5 µg/ml) in ICC/IF.

  • Paraffin embedded mouse lung tissue stained for TRPC6 with ab62461 (10 µg/ml) in immunohistochemical analysis.

  • K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 using ab62461 (2 µg/ml) in ICC.

References

This product has been referenced in:

  • Bulley S  et al. Arterial smooth muscle cell PKD2 (TRPP1) channels regulate systemic blood pressure. Elife 7:N/A (2018). Read more (PubMed: 30511640) »
  • Yang X  et al. Role of transient receptor potential channel 6 in the odontogenic differentiation of human dental pulp cells. Exp Ther Med 14:73-78 (2017). Read more (PubMed: 28672895) »
See all 6 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

I was not able to obtain the exact sequence of the immunogen, as it is considered proprietary by our source for this antibody. I did learn that the immunogen shares 92% identity with rat TRPC6. There are 13 contiguous identical amino acids out of 14.

However, the lab also reported that a western blot of rat heart was negative. This is a little surprising, given the high homology, but it was one test, and the result may have been unique to the batch that was tested.

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Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Mouse Cell (Podocyte)
Specification
Podocyte
Permeabilization
Yes - 0.1% triton-X
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 27 2012

Answer

Please find a new discount code that you can use to submit your Abreview for ab62461 in IF:

new discount code - 1*00%ABR-TBVX1, exp*



If there is anything else I can help you with, please let me know.

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Question
Answer

Thank you for contacting us. After consulting with the laboratory regarding ab62461, I have discovered that though the rat and mouse sequence are quite similar, they did test in rat and did not receive a positive signal.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you very much for your interest in our anti-TRPC6 antibodies.

While we do offer six products which work in human tissues, to our knowledge, none of these products have been tested in FACS on human cells. Therefore, I can offer a discount off a future purchase if you buy one of these now, test it in FACS and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY OR VALUE OFF OF NEXT ORDER.

The products are:
ab63038: https://www.abcam.com/TRPC6-antibody-mAbcam63038-ab63038.html
ab62461: https://www.abcam.com/TRPC6-antibody-Aminoterminal-end-ab62461.html
ab63000: https://www.abcam.com/TRPC6-antibody-ab63000.html
ab63009: https://www.abcam.com/TRPC6-antibody-ab63009.html
ab105845: https://www.abcam.com/TRPC6-antibody-3F2-H10-F2-ab105845.html
ab106840: https://www.abcam.com/TRPC6-antibody-ab106840.html

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test one of these products. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Test it in FACS.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY or PRODUCT ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for FACS, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Question
Answer

DISCOUNT CODE:************
Expiration date: ************

I am very pleased to hear you would like to accept our offer and test ab62461 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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