Overview

  • Product name

  • Description

    Rabbit polyclonal to TRPC6
  • Host species

    Rabbit
  • Specificity

    This antibody does potentially cross-react with TRPC3.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRPC6 (N terminal).
    Database link: Q9Y210
    (Peptide available as ab106953)

  • Positive control

    • WB: K562 cell lysate. IHC-P: Mouse lung tissue. ICC/IF: K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).

Target

  • Function

    Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
  • Tissue specificity

    Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
  • Involvement in disease

    Defects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities

    Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
    Contains 4 ANK repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • bZ1P14.9 antibody
    • FLJ11098 antibody
    • FLJ14863 antibody
    • FSGS2 antibody
    • MTRP6 antibody
    • Short transient receptor potential channel 6 antibody
    • si:rp71-1p14.9 antibody
    • Transient receptor potential cation channel subfamily C member 6 antibody
    • Transient receptor protein 6 antibody
    • TRP 6 antibody
    • TRP-6 antibody
    • TRP6 antibody
    • TRPC 6 antibody
    • Trpc6 antibody
    • TRPC6_HUMAN antibody
    • TRRP6 antibody
    see all

Images

  • Lane 1 : Anti-TRPC6 antibody (ab62461) at 0.5 µg/ml
    Lane 2 : Anti-TRPC6 antibody (ab62461) at 1 µg/ml

    All lanes : K562 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 106 kDa
    Observed band size: 106 kDa

  • K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 (red) using ab62461 (5 µg/ml) in ICC/IF.

  • Paraffin embedded mouse lung tissue stained for TRPC6 with ab62461 (10 µg/ml) in immunohistochemical analysis.

  • K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 using ab62461 (2 µg/ml) in ICC.

References

This product has been referenced in:

  • Zhang T  et al. Pseudoginsenoside-F11 Protects against Transient Cerebral Ischemia Injury in Rats Involving Repressing Calcium Overload. Neuroscience 411:86-104 (2019). Read more (PubMed: 31129202) »
  • Bulley S  et al. Arterial smooth muscle cell PKD2 (TRPP1) channels regulate systemic blood pressure. Elife 7:N/A (2018). Read more (PubMed: 30511640) »
See all 7 Publications for this product

Customer reviews and Q&As

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Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Mouse Cell (Podocyte)
Specification
Podocyte
Permeabilization
Yes - 0.1% triton-X
Fixative
Formaldehyde

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Verified customer

Submitted Jul 27 2012

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