Product nameAnti-TRPM2 antibody
See all TRPM2 primary antibodies
DescriptionRabbit polyclonal to TRPM2
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to TRPM2 aa 1200-1250.
Database link: 7226
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were affinity purified using the peptide immobilized on solid support.
Our Abpromise guarantee covers the use of ab11167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 18209485
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500. Predicted molecular weight: 17 kDa.|
|IP||Use at 1-4 µg/mg of lysate.|
FunctionNonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity.
Tissue specificityHighly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes.
Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily.
Contains 1 nudix hydrolase domain.
- Information by UniProt
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ICC/IF image of ab11167 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11167, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Foller M et al. TRPC6 contributes to the Ca(2+) leak of human erythrocytes. Cell Physiol Biochem 21:183-92 (2008). Flow Cyt ; Human . Read more (PubMed: 18209485) »
- Gasser A et al. Activation of T cell calcium influx by the second messenger ADP-ribose. J Biol Chem 281:2489-96 (2006). Read more (PubMed: 16316998) »