Overview

  • Product name
  • Description
    Rabbit polyclonal to TRPM2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to TRPM2 aa 1200-1250.
    Database link: 7226

Properties

Applications

Our Abpromise guarantee covers the use of ab11167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 18209485

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50.
WB 1/500. Predicted molecular weight: 17 kDa.
IP Use at 1-4 µg/mg of lysate.

Target

  • Function
    Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity.
  • Tissue specificity
    Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes.
  • Sequence similarities
    Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily.
    Contains 1 nudix hydrolase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EREG 1 antibody
    • EREG1 antibody
    • Estrogen responsive element associated gene 1 antibody
    • Estrogen responsive element associated gene 1 protein antibody
    • Estrogen-responsive element-associated gene 1 protein antibody
    • KNP 3 antibody
    • KNP3 antibody
    • Long transient receptor potential channel 2 antibody
    • LTRPC 2 antibody
    • LTrpC-2 antibody
    • LTrpC2 antibody
    • MGC133383 antibody
    • NUDT9H antibody
    • NUDT9L1 antibody
    • putative Ca2+ channel protein antibody
    • putative Ca2+ channel protein antibody
    • Transient receptor potential cation channel subfamily M member 2 antibody
    • Transient receptor potential channel 7 antibody
    • TRPC 7 antibody
    • TrpC7 antibody
    • TRPM 2 antibody
    • Trpm2 antibody
    • TRPM2_HUMAN antibody
    see all

Images

  • ICC/IF image of ab11167 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11167, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry. Based upon an alignment of human (Swiss-Prot entry O94759 ) and rat (TrEMBL entry Q5G856 ) TRPM2. The site for ab11168, which recognizes both TRPM2-L and TRPM2-S, is identical in human and rat. The site recognized by ab11167 is divergent between rat and human.

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Answer

Thank you for your enquiry. We don’t know about the rat sequence, but the site for ab11168, which recognizes both TRPM2-L and TRPM2-S, is identical in human and mouse. The site recognized by ab11167 is divergent between mouse and human. I hope this helps.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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