Overview

  • Product name
  • Description
    Rabbit polyclonal to TRPM2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-FoFr, ICC, WB, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, which represents a portion of the human transient receptor potential cation channel, subfamily M, member 2 encoded within exon 13 (LocusLink ID 7226).

  • Positive control
    • IHC-P: Rat brain tissue. WB: Jurkat membrane preparation. IP: Jurkat membrane preparation.

Properties

Applications

Our Abpromise guarantee covers the use of ab11168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.
ICC 1/50.
WB 1/300. Predicted molecular weight: 171 kDa.
IP Use at 1-4 µg/mg of lysate.

Target

  • Function
    Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity.
  • Tissue specificity
    Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes.
  • Sequence similarities
    Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily.
    Contains 1 nudix hydrolase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EREG 1 antibody
    • EREG1 antibody
    • Estrogen responsive element associated gene 1 antibody
    • Estrogen responsive element associated gene 1 protein antibody
    • Estrogen-responsive element-associated gene 1 protein antibody
    • KNP 3 antibody
    • KNP3 antibody
    • Long transient receptor potential channel 2 antibody
    • LTRPC 2 antibody
    • LTrpC-2 antibody
    • LTrpC2 antibody
    • MGC133383 antibody
    • NUDT9H antibody
    • NUDT9L1 antibody
    • putative Ca2+ channel protein antibody
    • putative Ca2+ channel protein antibody
    • Transient receptor potential cation channel subfamily M member 2 antibody
    • Transient receptor potential channel 7 antibody
    • TRPC 7 antibody
    • TrpC7 antibody
    • TRPM 2 antibody
    • Trpm2 antibody
    • TRPM2_HUMAN antibody
    see all

Images

  • All lanes : Anti-TRPM2 antibody (ab11168) at 0.1 µg

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) membrane preparation at 50 µg
    Lane 2 : Jurkat membrane preparation at 15 µg
    Lane 3 : Jurkat membrane preparation at 5 µg

    Developed using the ECL technique.

    Predicted band size: 171 kDa


    Exposure time: 3 minutes
  • IHC image of ab11168 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11168, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Luo X  et al. Selective inhibition of TRPM2 channel by two novel synthesized ADPR analogues. Chem Biol Drug Des 91:552-566 (2018). WB ; Human . Read more (PubMed: 29034580) »
  • Di A  et al. Role of the phagosomal redox-sensitive TRP channel TRPM2 in regulating bactericidal activity of macrophages. J Cell Sci 130:735-744 (2017). Read more (PubMed: 28082421) »
See all 8 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Neutrophil)
Permeabilization
No
Specification
Neutrophil
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Sep 25 2015

Answer

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. I look forward to receiving your reply. PS: Please include images for each antibody also. Images always helps in understanding the problem quickly.

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Answer

Thank you for your enquiry. There appears to be three human isoforms of this target (Swiss Prot). TRPM2-S should have a predicted KDa of 101 and TRPM2-L should have a predicted KDa of 180 (Swiss Prot).I have removed the predicted KDa of 17 from the data sheet as I believe this is a mistake, thank you for bringing this matter to my attention and best of luck with your research.

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Answer

Thank you for your enquiry. There appears to be three human isoforms of this target (Swiss Prot). TRPM2-S should have a predicted KDa of 101 and TRPM2-L should have a predicted KDa of 180 (Swiss Prot).I have removed the predicted KDa of 17 from the data sheet as I believe this is a mistake, thank you for bringing this matter to my attention and best of luck with your research.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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