Overview

  • Product name

  • Description

    Goat polyclonal to TRPM2
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TRPM2 aa 1139-1150 (internal sequence) (Cysteine residue). (NP_003298.1; NP_001307279.1; NP_001307280.1)
    Sequence:

    C-QQKQRPEQKIED


    Database link: O94759

  • Positive control

    • ICC/IF: A431 cells. Flow Cyt: Jurkat cells. IHC-P: Human pancreas and cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab240540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 3.75 µg/ml.

Steamed antigen retrieval with citrate buffer pH 6.

Target

  • Function

    Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity.
  • Tissue specificity

    Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes.
  • Sequence similarities

    Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily.
    Contains 1 nudix hydrolase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EREG 1 antibody
    • EREG1 antibody
    • Estrogen responsive element associated gene 1 antibody
    • Estrogen responsive element associated gene 1 protein antibody
    • Estrogen-responsive element-associated gene 1 protein antibody
    • KNP 3 antibody
    • KNP3 antibody
    • Long transient receptor potential channel 2 antibody
    • LTRPC 2 antibody
    • LTrpC-2 antibody
    • LTrpC2 antibody
    • MGC133383 antibody
    • NUDT9H antibody
    • NUDT9L1 antibody
    • putative Ca2+ channel protein antibody
    • putative Ca2+ channel protein antibody
    • Transient receptor potential cation channel subfamily M member 2 antibody
    • Transient receptor potential channel 7 antibody
    • TRPC 7 antibody
    • TrpC7 antibody
    • TRPM 2 antibody
    • Trpm2 antibody
    • TRPM2_HUMAN antibody
    see all

Images

  • Paraformaldehyde-fixed, 0.15% Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TRPM2 (green) using ab240540 at 10 μg/ml for 1 hour followed by an Alexa Fluor® 488-conjugated secondary antibody in ICC/IF. The nuclear counter stain is DAPI (blue).

    The negative control is unimmunized goat IgG at 10 μg/ml followed by an Alexa Fluor® 488-congugated secondary antibody.

  • Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling TRPM2 with ab240540 at 10 μg/ml (blue) compared to an unimmunized goat IgG negative control (black). The secondary antibody is AlexaFluor® 488-conjugated antibody at 1 μg/ml.

  • Paraffin-embedded human pancreas tissue stained for TRPM2 using ab240540 at 3.75 μg/ml in immunohistochemical analysis. AP staining.

  • Paraffin-embedded human cortex tissue stained for TRPM2 using ab240540 at 3.75 μg/ml in immunohistochemical analysis. AP staining.

References

ab240540 has not yet been referenced specifically in any publications.

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