Overview

  • Product name
    Anti-TRPM6 antibody
  • Description
    Guinea pig polyclonal to TRPM6
  • Host species
    Guinea pig
  • Tested applications
    Suitable for: IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    GCERDKNRSSLERHTRL

Properties

Applications

Our Abpromise guarantee covers the use of ab47017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/1000.

Target

  • Relevance
    TRPM6 is an essential ion channel and serine/threonine-protein kinase, and is crucial for magnesium homeostasis. TRPM6 also has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. The various isoforms of the type M6-kinase lack the ion channel region.
  • Cellular localization
    Multi-pass membrane protein.
  • Database links
  • Alternative names
    • CHAK2 antibody
    • Channel kinase 2 antibody
    • HMGX antibody
    • HOMG antibody
    • HSH antibody
    • Melastatin related TRP cation channel 6 antibody
    • Transient receptor potential cation channel subfamily M member 6 antibody
    see all

Images

  • Immunohistochemical staining of frozen rat kidney tissue, using ab47017. Secondary staining was carried out using a Cy3 conjugated antibody.

References

This product has been referenced in:
  • Ferioli S  et al. TRPM6 and TRPM7 differentially contribute to the relief of heteromeric TRPM6/7 channels from inhibition by cytosolic Mg2+and Mg·ATP. Sci Rep 7:8806 (2017). Read more (PubMed: 28821869) »
  • Boyden LM  et al. Mutations in kelch-like 3 and cullin 3 cause hypertension and electrolyte abnormalities. Nature 482:98-102 (2012). Read more (PubMed: 22266938) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply and your patience with this response.

I heard back from the lab in Australia, and unfortunately none of our anti-TRPM6 antibodies (ab47017, ab62990, ab63089, ab72153, ab74850, ab77499, ab79227) show significant homology with bovine. Therefore, we wouldn't expect any of these antibodies to work in bovine. I am very sorry that we can't provide you an antibody for bovine in this case.

But for ab72155, I will send you a discount code as this antibody should work in bovine due to high sequence homology.

I am very pleased to hear you would like to test ab72155 in bovine. This code will give you £227.00 off your next order before the expiration date. To redeem this offer, please submit an Abreview for bovine and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

Read More

Question
Answer

Thank you for your inquiry.

I just wanted to give you an update.

We have 7 anti-TRPM6 antibodies in the catalogue. We wouldn't expect ab47017 to work in bovine, because the immunogen sequence really does not show any homology with bovine.

For the other 6 antibodies (ab62990, ab63089, ab72153, ab74850, ab77499, ab79227), these all come from the same lab in Australia and that lab is a little "tight-lipped" on their immunogen sequences. I've sent them an email to see if they can BLAST the immunogens against bovine for us, but right now it's 11:30pm there so I don't expect to have an answer for you at least until tomorrow.

I really apologize for the delay, but as soon as I hear back from the lab regarding any significant homology with bovine, I will let you know.

Have a great day!

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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