Overview

  • Product name

    Anti-TRPM7 + TRPM6 antibody [EPR4582]
  • Description

    Rabbit monoclonal [EPR4582] to TRPM7 + TRPM6
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 86% homology with TRPM6 (ten amino acid stretch with 100% homology). ab109438 has been shown to cross react with TRPM6 in WB.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TRPM7 aa 1800-1900 (C terminal).
    (Peptide available as ab195344)

  • Positive control

    • HuT-78, JAR, and HepG2 whole cell lysate (ab7900). HeLa cells and cell lysates.
  • General notes

    We have preliminary internal testing data to indicate this antibody may not react with mouse and rat species. Please contact us for more information

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 213 kDa.Can be blocked with TRPM7 peptide (ab195344).
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    Images

    • All lanes : Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : HuT-78 cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 213 kDa

    • All lanes : Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/500 dilution

      Lane 1 : His-Tagged human TRPM6 (aa 1755 to 2022) recombinant protein with 5% NFDM/TBST
      Lane 2 : His-Tagged human TRPM7 (aa 1599 to 1986) recombinant protein with 5% NFDM/TBST

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 213 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 seconds
    • Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/1000 dilution (purified) + JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 213 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    References

    This product has been referenced in:

    • Liu L  et al. TRPM7 promotes the epithelial-mesenchymal transition in ovarian cancer through the calcium-related PI3K / AKT oncogenic signaling. J Exp Clin Cancer Res 38:106 (2019). Read more (PubMed: 30819230) »
    • Thuringer D  et al. The vesicular transfer of CLIC1 from glioblastoma to microvascular endothelial cells requires TRPM7. Oncotarget 9:33302-33311 (2018). Read more (PubMed: 30279961) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Pig Cell lysate - whole cell (Cardiac Fibroblasts)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Cardiac Fibroblasts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted May 17 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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