Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4582] to TRPM7 + TRPM6 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Product nameAnti-TRPM7 + TRPM6 antibody [EPR4582] - BSA and Azide free
DescriptionRabbit monoclonal [EPR4582] to TRPM7 + TRPM6 - BSA and Azide free
The immunogen used for this product shares 86% homology with TRPM6 (ten amino acid stretch with 100% homology). ab109438 (The same clone in a diffrent buffer) has been shown to cross react with TRPM6 in WB.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
- WB: JAR whole cell lysate.
Ab232455 is the carrier-free version of ab109438. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232455 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab232455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 213 kDa.|
Cellular localizationTRPM7: Membrane. TRPM6: Multi-pass membrane protein.
Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/1000 dilution + JAR (human placenta choriocarcinoma cell line) whole cell lysate at 15 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 213 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109438).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232455 has been referenced in 1 publication.
- Wan J et al. TRPM7 Induces Mechanistic Target of Rap1b Through the Downregulation of miR-28-5p in Glioma Proliferation and Invasion. Front Oncol 9:1413 (2019). PubMed: 31921670