Overview

  • Product name

  • Description

    Goat polyclonal to TRPM7
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ELISA, ICC/IF, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Crayfish
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide: TKESESTNSVRLML, corresponding to C terminal amino acids 1852-1865 of Human TRPM7.

  • Positive control

    • Daudi lysate
  • General notes

    In response to customer feedback and in-house testing the latest lot doesn't pass the WB quality testing so we have decided to remove the application for now. Please contact technical@abcam.com for more details.

     

    Please see reference He et al for details of the size of the protein detected by SDS-PAGE. Principal Names: TRPM7; transient receptor potential cation channel, subfamily M, member 7; CHAK; CHAK1; LTRPC7; FLJ20117; TRP-PLIK; LTRPC ion channel family member 7; homolog of mouse transient receptor potential-phospholipase C-interacting kinase CHaK hypothetical protein. Official Gene Symbol - TRPM7 GenBank Accession Number – XP_030709 LocusLink ID - 54822 (human).

     Can be blocked with Human TRPM7 peptide (ab22777).

     

Properties

Applications

Our Abpromise guarantee covers the use of ab729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 21183474
IHC-Fr 1/1000. PubMed: 16285951
ELISA 1/16000.
ICC/IF Use at an assay dependent concentration. PubMed: 16285951
IHC-FoFr Use at an assay dependent concentration. PubMed: 19734892

Target

  • Function

    Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
  • Involvement in disease

    Defects in TRPM7 are a cause of susceptibility to amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 (ALS-PDC1) [MIM:105500]; also called amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam or Guam disease. Amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 is a neurodegenerative disorder characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. Both diseases are known to occur in the same kindred, the same sibship and even the same individual.
  • Sequence similarities

    In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
    In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
    Contains 1 alpha-type protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALSPDC antibody
    • CHAK antibody
    • CHAK1 antibody
    • Channel kinase 1 antibody
    • Channel-kinase 1 antibody
    • Long transient receptor potential channel 7 antibody
    • LTrpC-7 antibody
    • LTrpC7 antibody
    • Transient receptor potential cation channel subfamily M member 7 antibody
    • TRP PLIK antibody
    • TRPM7 antibody
    • TRPM7_HUMAN antibody
    see all

References

This product has been referenced in:

See all 19 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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1-2 of 2 Abreviews

Application
Western blot
Sample
Crayfish Cell lysate - whole cell (crayfish nerve chord)
Loading amount
10 µg
Specification
crayfish nerve chord
Gel Running Conditions
Reduced Denaturing (4-15 % BioRad precast gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Lorelei Silverman Gavrila

Verified customer

Submitted Sep 09 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Crayfish Cell (phasic and tonic axons and butons)
Specification
phasic and tonic axons and butons
Fixative
Paraformaldehyde
Permeabilization
Yes - TritonX-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rm°C

Dr. Lorelei Silverman Gavrila

Verified customer

Submitted Sep 08 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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