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I followed your suggestions to perform western blot by using 3% BSA for blocking overnight and washing with TBST (0.1% Tween 20). Please find the Attached file that shows the result of western blot. The adjustment of the experiment condictions did not improve my results much.
Asked on Feb 07 2007
Thank you for your e-mail to Hugh who is away today. I'm very sorry to hear you are still experiencing lots of non specific bands with ab3243 even after the protocol modifications. I would be happy to offer you a replacement vial or refund if you purchased the antibody in the last 3 months. Can you please confirm your order details and what you would prefer? I look forward to hearing from you to arrange this,
Answered on Feb 08 2007