Anti-TRPM8 antibody (ab85617)

Reviews (1)Q&A (2)References (1)
Western blot - Anti-TRPM8 antibody (ab85617)

    Key features and details

    • Rabbit polyclonal to TRPM8
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-TRPM8 antibody
      See all TRPM8 primary antibodies

    • Description

      Rabbit polyclonal to TRPM8

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide corresponding to Human TRPM8 aa 250-350 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab95862)

    • Positive control

      • This antibody gave a positive signal in the following human whole cell lysates: HeLa; PC3.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab85617 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rabbit
    Dog
    Monkey
    Baboon
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).

    Target

    • Function

      Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.

    • Tissue specificity

      Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.

    • Sequence similarities

      Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Long transient receptor potential channel 6 antibody
        • LTrpC-6 antibody
        • LTrpC6 antibody
        • MGC2849 antibody
        • Short form of the TRPM8 cationic channel antibody
        • Transient receptor potential cation channel subfamily M member 8 antibody
        • Transient receptor potential p8 antibody
        • transient receptor potential-p8 antibody
        • Trp p8 antibody
        • Trp-p8 antibody
        • Trpm8 antibody
        • TRPM8_HUMAN antibody
        • TRPP8 antibody
        see all

      Images

      • Western blot - Anti-TRPM8 antibody (ab85617)
        Western blot - Anti-TRPM8 antibody (ab85617)
        All lanes : Anti-TRPM8 antibody (ab85617) at 1 µg/ml

        Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 127 kDa
        Observed band size: 140 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 32 kDa, 87 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 2 minutes

      References (1)

      Publishing research using ab85617? Please let us know so that we can cite the reference in this datasheet.

      ab85617 has been referenced in 1 publication.

      • Bidaux G  et al. Regulation of Activity of Transient Receptor Potential Melastatin 8 (TRPM8) Channel by Its Short Isoforms. J Biol Chem 287:2948-62 (2012). WB ; Human . PubMed: 22128173

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Immunoprecipitation abreview for Anti-TRPM8 antibody

       Poor
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (stably human TRPM8-transfected HEK293 cells)
      Total protein in input
      500 µg
      Specification
      stably human TRPM8-transfected HEK293 cells
      Immuno-precipitation step
      Protein A
      Read More

      Miss. Larisa Maier

      Verified customer

      Submitted May 15 2013

      Question

      Bonjour,

      Ce produit ne fonctionne pas chez la souris.

      Il fonctionne néanmoins très bien western-blot sur la forme humain. La spécificité n'est par contre pas claire du tout en IF et IHC sur des cellules humaines.

      Nous garderons ce lot pour le Wb mais c'est aussi une des raisons qui fait que je souhaite tester une gamme plus large d'anticorps.

      Merci,

      Read More
      Answer

      Merci beaucoup aussi pourcet émail etpour votre retour sur cet anticorps.

      Je suis désolée quecet anticorpsne fonctionne pas dans les autres applications. Comme nous ne l'avions pas testé, nous ne pouvions pas non plus le garantir. Néanmoins, pour partager votre expérience sur cet anticorps avec les autres chercheurs et recevoir des Abpoints de nous, je vous invite à écrire des Abreviews sur ce produit. Effectivement, une Abreview c'est un feedback court des utilisateurs sur l'utilisation d'un produit surune certain espèce ou application, et vous permets d'accumuler des points soit pour des remises soit pour des bons Amazon. Veuillez trouver plus d'information sous le site https://www.abcam.com/abreviews

      J'espère que cette information vous est utile. N'hésitez pas à nous recontacter si vous avez des questions ou soucis à ce sujet.

      Read More

      Question

      Extrait d' émail: ab85617, produit un signal aspécifique en immunohisto sur la souris. Je ne suis pas non plus convaincue de sa spécificité conte les séquences humaines en IF bien qu'il fonctionne proprement en WB

      Read More
      Answer

      Je suis désolée d'apprendre que ab85617 ne vous donne pas de bons résultats en ni en Western blot ni en IF.

      Nous aimerons bien investiguer ceci. Veuillez trouver ci-joint un questionnaire qui nous permet de regrouper le plus d'informations possible sur le protocole que vous avez utilisé avec cet anticorps.
      Celui-ci nous permettra de vous fournir la meilleure assistance technique.

      Si nous concluons que notre produit est fautif et ne fonctionne pas comme explicité par notre fiche technique, nous serons ravis de mettre en place l'envoi d'un tube de remplacement gratuit ou d'un remboursement (sous réserve d'une commande ultérieure de 180 jours).

      Je vous remercie par avance de nous envoyer votre protocole.

      Read More

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