Overview

  • Product name

  • Description

    Rabbit polyclonal to TRPM8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Dog, Monkey, Baboon
  • Immunogen

    Synthetic peptide corresponding to Human TRPM8 aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab95862)

  • Positive control

    • This antibody gave a positive signal in the following human whole cell lysates: HeLa; PC3.

Properties

Applications

Our Abpromise guarantee covers the use of ab85617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).

Target

  • Function

    Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
  • Tissue specificity

    Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
  • Sequence similarities

    Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Long transient receptor potential channel 6 antibody
    • LTrpC-6 antibody
    • LTrpC6 antibody
    • MGC2849 antibody
    • Short form of the TRPM8 cationic channel antibody
    • Transient receptor potential cation channel subfamily M member 8 antibody
    • Transient receptor potential p8 antibody
    • transient receptor potential-p8 antibody
    • Trp p8 antibody
    • Trp-p8 antibody
    • Trpm8 antibody
    • TRPM8_HUMAN antibody
    • TRPP8 antibody
    see all

Images

  • All lanes : Anti-TRPM8 antibody (ab85617) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 127 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 32 kDa, 87 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes

References

This product has been referenced in:

  • Bidaux G  et al. Regulation of Activity of Transient Receptor Potential Melastatin 8 (TRPM8) Channel by Its Short Isoforms. J Biol Chem 287:2948-62 (2012). WB ; Human . Read more (PubMed: 22128173) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (stably human TRPM8-transfected HEK293 cells)
Total protein in input
500 µg
Specification
stably human TRPM8-transfected HEK293 cells
Immuno-precipitation step
Protein A

Miss. Larisa Maier

Verified customer

Submitted May 15 2013

Answer

Merci beaucoup aussi pourcet émail etpour votre retour sur cet anticorps.

Je suis désolée quecet anticorpsne fonctionne pas dans les autres applications. Comme nous ne l'avions pas testé, nous ne pouvions pas non plus le garantir. Néanmoins, pour partager votre expérience sur cet anticorps avec les autres chercheurs et recevoir des Abpoints de nous, je vous invite à écrire des Abreviews sur ce produit. Effectivement, une Abreview c'est un feedback court des utilisateurs sur l'utilisation d'un produit surune certain espèce ou application, et vous permets d'accumuler des points soit pour des remises soit pour des bons Amazon. Veuillez trouver plus d'information sous le site https://www.abcam.com/abreviews

J'espère que cette information vous est utile. N'hésitez pas à nous recontacter si vous avez des questions ou soucis à ce sujet.

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Answer

Je suis désolée d'apprendre que ab85617 ne vous donne pas de bons résultats en ni en Western blot ni en IF.

Nous aimerons bien investiguer ceci. Veuillez trouver ci-joint un questionnaire qui nous permet de regrouper le plus d'informations possible sur le protocole que vous avez utilisé avec cet anticorps.
Celui-ci nous permettra de vous fournir la meilleure assistance technique.

Si nous concluons que notre produit est fautif et ne fonctionne pas comme explicité par notre fiche technique, nous serons ravis de mettre en place l'envoi d'un tube de remplacement gratuit ou d'un remboursement (sous réserve d'une commande ultérieure de 180 jours).

Je vous remercie par avance de nous envoyer votre protocole.

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