- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-TRPM8 antibody
See all TRPM8 primary antibodies -
DescriptionRabbit polyclonal to TRPM8
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Dog, Monkey, Baboon
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human TRPM8.
(Peptide available as ab95862.)
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Positive control
- This antibody gave a positive signal in the following human whole cell lysates: HeLa; PC3.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab85617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa). |
Target
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FunctionReceptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
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Tissue specificityExpressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
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Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 79054 Human
- Entrez Gene: 171382 Mouse
- Omim: 606678 Human
- SwissProt: Q7Z2W7 Human
- SwissProt: Q8R4D5 Mouse
- Unigene: 366053 Human
- Unigene: 218753 Mouse
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Alternative names
- Long transient receptor potential channel 6 antibody
- LTrpC-6 antibody
- LTrpC6 antibody
see all
Images
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Western blot - Anti-TRPM8 antibody (ab85617)All lanes : Anti-TRPM8 antibody (ab85617) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 140 kDa (why is the actual band size different from the predicted?)
Additional bands at: 32 kDa, 87 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Protocols
References
This product has been referenced in:
- Bidaux G et al. Regulation of Activity of Transient Receptor Potential Melastatin 8 (TRPM8) Channel by Its Short Isoforms. J Biol Chem 287:2948-62 (2012). WB ; Human . Read more (PubMed: 22128173) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"