Anti-TRPM8 antibody (ab85617)
Key features and details
- Rabbit polyclonal to TRPM8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRPM8 antibody
See all TRPM8 primary antibodies -
Description
Rabbit polyclonal to TRPM8 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Dog, Monkey, Baboon -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following human whole cell lysates: HeLa; PC3.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa). |
Target
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Function
Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. -
Tissue specificity
Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors. -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 79054 Human
- Entrez Gene: 171382 Mouse
- Omim: 606678 Human
- SwissProt: Q7Z2W7 Human
- SwissProt: Q8R4D5 Mouse
- Unigene: 366053 Human
- Unigene: 218753 Mouse
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Alternative names
- Long transient receptor potential channel 6 antibody
- LTrpC-6 antibody
- LTrpC6 antibody
see all
Images
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All lanes : Anti-TRPM8 antibody (ab85617) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 87 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab85617 has been referenced in 2 publications.
- Amato A et al. TRPM8 Channel Activation Reduces the Spontaneous Contractions in Human Distal Colon. Int J Mol Sci 21:N/A (2020). PubMed: 32751347
- Bidaux G et al. Regulation of Activity of Transient Receptor Potential Melastatin 8 (TRPM8) Channel by Its Short Isoforms. J Biol Chem 287:2948-62 (2012). WB ; Human . PubMed: 22128173