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Signal Transduction Metabolism Plasma Membrane Channels
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RecombinantRabMAb

Recombinant Anti-TRPM8 antibody [EPR4196(2)] (ab109308)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (8)

Product price, shipping and contact information

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Abpromise

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Western blot - Anti-TRPM8 antibody [EPR4196(2)] (ab109308)
  • Anti-TRPM8 antibody [EPR4196(2)] (ab109308)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4196(2)] to TRPM8
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-TRPM8 antibody [EPR4196(2)]
    See all TRPM8 primary antibodies
  • Description

    Rabbit monoclonal [EPR4196(2)] to TRPM8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in the extracellular domain of Human TRPM8.

  • Positive control

    • HT-1376, LnCaP and A549 cell lysates
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4196(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-TRPM8 antibody [EPR4196(2)] - BSA and Azide free (ab185529)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • TRPM8 peptide (ab187961)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab109308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa).Can be blocked with TRPM8 peptide (ab187961).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
    • Tissue specificity

      Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
    • Sequence similarities

      Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q7Z2W7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 79054 Human
      • Entrez Gene: 171382 Mouse
      • Omim: 606678 Human
      • SwissProt: Q7Z2W7 Human
      • SwissProt: Q8R4D5 Mouse
      • Unigene: 366053 Human
      • Unigene: 218753 Mouse
      • Alternative names

        • Long transient receptor potential channel 6 antibody
        • LTrpC-6 antibody
        • LTrpC6 antibody
        • MGC2849 antibody
        • Short form of the TRPM8 cationic channel antibody
        • Transient receptor potential cation channel subfamily M member 8 antibody
        • Transient receptor potential p8 antibody
        • transient receptor potential-p8 antibody
        • Trp p8 antibody
        • Trp-p8 antibody
        • Trpm8 antibody
        • TRPM8_HUMAN antibody
        • TRPP8 antibody
        see all

      Images

      • Western blot - Anti-TRPM8 antibody [EPR4196(2)] (ab109308)
        Western blot - Anti-TRPM8 antibody [EPR4196(2)] (ab109308)
        All lanes : Anti-TRPM8 antibody [EPR4196(2)] (ab109308) at 1/1000 dilution

        Lane 1 : HT-1376 cell lysates
        Lane 2 : LnCaP cell lysates
        Lane 3 : A549 cell lysates

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 128 kDa
        Observed band size: 130 kDa
        why is the actual band size different from the predicted?

      • Anti-TRPM8 antibody [EPR4196(2)] (ab109308)
        Anti-TRPM8 antibody [EPR4196(2)] (ab109308)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (8)

      Publishing research using ab109308? Please let us know so that we can cite the reference in this datasheet.

      ab109308 has been referenced in 8 publications.

      • Grolez GP  et al. TRPM8-androgen receptor association within lipid rafts promotes prostate cancer cell migration. Cell Death Dis 10:652 (2019). PubMed: 31501416
      • Bidaux G  et al. 4TM-TRPM8 channels are new gatekeepers of the ER-mitochondria Ca2+ transfer. Biochim Biophys Acta 1865:981-994 (2018). WB ; Human . PubMed: 29678654
      • Genova T  et al. TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1. J Cell Biol 216:2107-2130 (2017). PubMed: 28550110
      • Borowiec AS  et al. Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock-induced oxidation. FASEB J N/A:N/A (2016). PubMed: 27317670
      • Bidaux G  et al. Targeting of short TRPM8 isoforms induces 4TM-TRPM8-dependent apoptosis in prostate cancer cells. Oncotarget 7:29063-80 (2016). WB . PubMed: 27074561
      • Bidaux G  et al. Epidermal TRPM8 channel isoform controls the balance between keratinocyte proliferation and differentiation in a cold-dependent manner. Proc Natl Acad Sci U S A 112:E3345-54 (2015). PubMed: 26080404
      • Bidaux G  et al. Functional and Modeling Studies of the Transmembrane Region of the TRPM8 Channel. Biophys J 109:1840-51 (2015). PubMed: 26536261
      • Winking M  et al. Importance of a conserved sequence motif in transmembrane segment S3 for the gating of human TRPM8 and TRPM2. PLoS One 7:e49877 (2012). WB ; Human . PubMed: 23185472

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-6 of 6 Abreviews or Q&A

      Question

      ab109308: Immunogen sequence

      Read More

      Abcam community

      Verified customer

      Asked on Feb 22 2013

      Answer

      The immunogen sequence for this antibody is CVELDEHNLPRFPEW and range is 940-954.

      Read More

      Abcam Scientific Support

      Answered on Feb 22 2013

      Question

      Hello, Under what conditions do you recommend using this Antibody for Western Blotting? Can we follow a standard Denaturing/Reducing protocol, or is this Antibody only tested for whole protein?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 12 2013

      Answer

      Thank you for contacting us.

      Yes, we recommend running the WB in reduced and denatured conditions.

      You can find the recommended protocol here: http://www.epitomics.com/products/support/wb

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Feb 12 2013

      Question

      Customer inquring about IHC-P on human tissue for detecting BMPR2. Also for detecting TRPM8 via ICC and western blot. Customer asked for protocol tips and antibody suggestions.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 28 2012

      Answer

      Thank you for contacting Abcam.

      We spoke earlier about two antibodies:
      anti-BMPR2 ab78422 (https://www.abcam.com/bmpr2-antibody-mm0060-9a10-ab78422.html)
      anti-TRPM8 ab109308 (https://www.abcam.com/trpm8-antibody-epr41962-ab109308.html)

      You mentioned you were interested in testing ab78422 in mouse via IHC-P prior to using the antibody in human samples. Since this would be a mouse on mouse stain, there will be a high level of background due to the mouse antibody binding to native IgG's present in the mouse tissue. Also, our AbTrial program does not apply to mouse on mouse applications. Thus, I highly recommend testing the antibody in your human sections, perhaps sections that are not as crucial to your experiment.

      Additionally, purchase of ab78422 would entitle you to a free rabbit monoclonal antibody through our RabMab promotion. Details of this promotion are given below:
      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Thus, you would be able to receive ab109308, the anti-TRPM8 RabMab that we looked at together earlier with the purchase of ab78422.

      We also have anti-mouse and anti-rabbit secondary antibodies that could be used for ab78422 and ab109308, respectively.
      anti-mouse: https://www.abcam.com/Search?AppliedFacets.FacetProductType=Secondary+antibodies&IsFacetsOnly=False&PageNumber=1&Keywords[0]=anti-mouse&X-Requested-With=XMLHttpRequest
      anti-rabbit:https://www.abcam.com/Search?Keywords=anti-rabbit&fReset=1&pt=7&source=TopSearch&btnSearch=Search

      We also discussed some IHC-P protocol information. I've attached our protocol book which includes IHC-P protocol information as well as other applications. Specifically for ab78422, we discussed attempting a proteinase K enzymatic antigen retrieval. Information regarding a proteinase K antigen retrieval is given below. I also recommend primary antibody dilutions of 1/50, 1/100, and 1/200 to see which dilution gives the best signal to background ratio.
      Proteinase K antigen retrieval information: http://www.ihcworld.com/_protocols/epitope_retrieval/proteinase-k.htm

      We also discussed the western blot bands that you were seeing for TRPM8, with 2 bands appearing (1 at the expected 130 kDa and 1 at 60 kDa). Since the TRPM8 protein is a homotetramer, the 60 kDa band is most likely a homodimer composed of half of the TRPM8 protein. In order to reduce the likelihood of this lower weight band occurring, you can also attempt denaturation in SDS at 70oC for 5-10 minutes rather than boiling at 95-100oC.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Nov 28 2012

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TRPM8 antibody [EPR4196(2)]

      Poor
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Prostate)
      Specification
      Prostate
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate
      Permeabilization
      Yes - Tween 0.1%
      Blocking step
      Serum as blocking agent for 45 minute(s) · Concentration: 1.2% · Temperature: 37°C
      Read More

      Dr. Gabriel Bidaux

      Verified customer

      Submitted Aug 16 2012

      Question

      séquences immunogènes pour plusieurs anti-TRPM8.
      utilisation WB, ICC/IF, IHC, IEM, IP, espèces étudiées : humain et souris.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 28 2012

      Answer

      Merci de votre intérêt pour les anticorps Abcam anti-TRPM8.

      Comme expliqué par téléphone,certains anti-TRPM8 n’ont pas encore été testés chez la souris et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez testerun anticorps chez une espèce ou dans une application qui n'est pas sur la fiche technique, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, un anticorps primaire vous sera offert gratuitement.

      Etapes à suivre :

      1. Nous confirmer que vous souhaiteriez testerun anticorps Abcam (préciser l'espèce ou l'application)afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de l'anticorps à tester

      2. Acheterl'anticorps par téléphone, fax ou internet (www.abcam.com)

      3. Le tester

      4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

      5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
      Même si l'anticorps ne pas fonctionne pas dans la nouvelle application ou espèce, après soumission de votre Abreview, vous recevrez quand même votre prochain anticorps gratuitement.

      N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

      Termes et Conditions : www.abcam.com/collaborationdiscount.

      Je vous recontacterai dès que j'aurai reçu les informations sur lesimmunogènes desanti-TRPM8 dont nousavons parlé.

      Read More

      Abcam Scientific Support

      Answered on Feb 28 2012

      Question

      Thank you very much, could you then please send me a discounted quote to test the following antibodies: ab71245, ab74846,ab104569, ab109308?

      Read More

      Abcam community

      Verified customer

      Asked on Sep 14 2011

      Answer

      Thank you for your interest in these antibodies. Without a discount the prices range from €360.00 to €370.00. A bulk order discount of approximately 5% is available for orders of 5 units or more of 5 different antibodies. The discount my colleague mentioned in an earlier e-mail is not a discount per se, but an offer of a free antibody in exchange for data from testing of an antibody that has been purchased already. Further, the offer is only available if the data is from a previously untested species or application (which are not covered by our guarantee). For example: Antibody ab74846 has been tested on human samples in several applications: IHC on frozen tissue (IHC-Fr), IHC on paraffin-embedded tissue (IHC-P) and western blotting (WB). Click here (or use the following: https://www.abcam.com/TRPM8-antibody-ab74846.html). If your samples are other than human (e.g., mouse, rat, etc.) or if the application you want to use the antibody for is other than IHC or WB (e.g., immunoprecipitation or ELISA, etc.), our guarantee of a replacement or a credit/refund if the antibody fails does not apply. However, if you send us your data, whether positive or negative, and you have a "discount" code, then the code will be active when we receive your data, and you can use it to receive another antibody for free when you place an order, for instance any of the other TRPM8 antibodies. I will need to know how you intend to use the antibody before I issue the discount code (or codes, if you want to test more than one antibody). I hope this makes sense. Please let me know if you have any questions. I look forward to your reply.

      Read More

      Abcam Scientific Support

      Answered on Sep 14 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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