Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4196(2)] to TRPM8
- Suitable for: WB
- Reacts with: Mouse, Human
Product nameAnti-TRPM8 antibody [EPR4196(2)]
See all TRPM8 primary antibodies
DescriptionRabbit monoclonal [EPR4196(2)] to TRPM8
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide, corresponding to residues in the extracellular domain of Human TRPM8.
- HT-1376, LnCaP and A549 cell lysates
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab109308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa).Can be blocked with TRPM8 peptide (ab187961).|
FunctionReceptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
Tissue specificityExpressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
- Information by UniProt
- Long transient receptor potential channel 6 antibody
- LTrpC-6 antibody
- LTrpC6 antibody
All lanes : Anti-TRPM8 antibody [EPR4196(2)] (ab109308) at 1/1000 dilution
Lane 1 : HT-1376 cell lysates
Lane 2 : LnCaP cell lysates
Lane 3 : A549 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 128 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
ab109308 has been referenced in 8 publications.
- Grolez GP et al. TRPM8-androgen receptor association within lipid rafts promotes prostate cancer cell migration. Cell Death Dis 10:652 (2019). PubMed: 31501416
- Bidaux G et al. 4TM-TRPM8 channels are new gatekeepers of the ER-mitochondria Ca2+ transfer. Biochim Biophys Acta 1865:981-994 (2018). WB ; Human . PubMed: 29678654
- Genova T et al. TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1. J Cell Biol 216:2107-2130 (2017). PubMed: 28550110
- Borowiec AS et al. Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock-induced oxidation. FASEB J N/A:N/A (2016). PubMed: 27317670
- Bidaux G et al. Targeting of short TRPM8 isoforms induces 4TM-TRPM8-dependent apoptosis in prostate cancer cells. Oncotarget 7:29063-80 (2016). WB . PubMed: 27074561
- Bidaux G et al. Epidermal TRPM8 channel isoform controls the balance between keratinocyte proliferation and differentiation in a cold-dependent manner. Proc Natl Acad Sci U S A 112:E3345-54 (2015). PubMed: 26080404
- Bidaux G et al. Functional and Modeling Studies of the Transmembrane Region of the TRPM8 Channel. Biophys J 109:1840-51 (2015). PubMed: 26536261
- Winking M et al. Importance of a conserved sequence motif in transmembrane segment S3 for the gating of human TRPM8 and TRPM2. PLoS One 7:e49877 (2012). WB ; Human . PubMed: 23185472