Anti-TRPM8 antibody [EPR4196(2)] (ab109308)
- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-TRPM8 antibody [EPR4196(2)]
See all TRPM8 primary antibodies -
DescriptionRabbit monoclonal [EPR4196(2)] to TRPM8
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Host speciesRabbit
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Tested applicationsSuitable for: ICC, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide, corresponding to residues in the extracellular domain of Human TRPM8.
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Positive control
- HT-1376, LnCaP and A549 cell lysates
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4196(2)
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab109308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/250 - 1/500. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa).Can be blocked with TRPM8 peptide (ab187961). |
Target
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FunctionReceptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
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Tissue specificityExpressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
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Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 79054 Human
- Entrez Gene: 171382 Mouse
- Omim: 606678 Human
- SwissProt: Q7Z2W7 Human
- SwissProt: Q8R4D5 Mouse
- Unigene: 366053 Human
- Unigene: 218753 Mouse
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Alternative names
- Long transient receptor potential channel 6 antibody
- LTrpC-6 antibody
- LTrpC6 antibody
see all
Images
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![Western blot - Anti-TRPM8 antibody [EPR4196(2)] (ab109308)](https://www.abcam.com/ps/products/109/ab109308/Images/TRPM8-Primary-antibodies-ab109308-1.jpg)
Western blot - Anti-TRPM8 antibody [EPR4196(2)] (ab109308)All lanes : Anti-TRPM8 antibody [EPR4196(2)] (ab109308) at 1/1000 dilution
Lane 1 : HT-1376 cell lysates
Lane 2 : LnCaP cell lysates
Lane 3 : A549 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 128 kDa
Observed band size: 130 kDa (why is the actual band size different from the predicted?)
References
This product has been referenced in:
- Bidaux G et al. 4TM-TRPM8 channels are new gatekeepers of the ER-mitochondria Ca2+ transfer. Biochim Biophys Acta 1865:981-994 (2018). WB ; Human . Read more (PubMed: 29678654) »
- Genova T et al. TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1. J Cell Biol 216:2107-2130 (2017). Read more (PubMed: 28550110) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"