Recombinant Anti-TRPM8 antibody [EPR4196(2)] (ab109308)

The immunogen sequence for this antibody is CVELDEHNLPRFPEW and range is 940-954.

Thank you for contacting us.

Yes, we recommend running the WB in reduced and denatured conditions.

You can find the recommended protocol here: http://www.epitomics.com/products/support/wb

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting Abcam.

We spoke earlier about two antibodies:
anti-BMPR2 ab78422 (https://www.abcam.com/bmpr2-antibody-mm0060-9a10-ab78422.html)
anti-TRPM8 ab109308 (https://www.abcam.com/trpm8-antibody-epr41962-ab109308.html)

You mentioned you were interested in testing ab78422 in mouse via IHC-P prior to using the antibody in human samples. Since this would be a mouse on mouse stain, there will be a high level of background due to the mouse antibody binding to native IgG's present in the mouse tissue. Also, our AbTrial program does not apply to mouse on mouse applications. Thus, I highly recommend testing the antibody in your human sections, perhaps sections that are not as crucial to your experiment.

Additionally, purchase of ab78422 would entitle you to a free rabbit monoclonal antibody through our RabMab promotion. Details of this promotion are given below:
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thus, you would be able to receive ab109308, the anti-TRPM8 RabMab that we looked at together earlier with the purchase of ab78422.

We also have anti-mouse and anti-rabbit secondary antibodies that could be used for ab78422 and ab109308, respectively.
anti-mouse: https://www.abcam.com/Search?AppliedFacets.FacetProductType=Secondary+antibodies&IsFacetsOnly=False&PageNumber=1&Keywords[0]=anti-mouse&X-Requested-With=XMLHttpRequest
anti-rabbit:https://www.abcam.com/Search?Keywords=anti-rabbit&fReset=1&pt=7&source=TopSearch&btnSearch=Search

We also discussed some IHC-P protocol information. I've attached our protocol book which includes IHC-P protocol information as well as other applications. Specifically for ab78422, we discussed attempting a proteinase K enzymatic antigen retrieval. Information regarding a proteinase K antigen retrieval is given below. I also recommend primary antibody dilutions of 1/50, 1/100, and 1/200 to see which dilution gives the best signal to background ratio.
Proteinase K antigen retrieval information: http://www.ihcworld.com/_protocols/epitope_retrieval/proteinase-k.htm

We also discussed the western blot bands that you were seeing for TRPM8, with 2 bands appearing (1 at the expected 130 kDa and 1 at 60 kDa). Since the TRPM8 protein is a homotetramer, the 60 kDa band is most likely a homodimer composed of half of the TRPM8 protein. In order to reduce the likelihood of this lower weight band occurring, you can also attempt denaturation in SDS at 70oC for 5-10 minutes rather than boiling at 95-100oC.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Merci de votre intérêt pour les anticorps Abcam anti-TRPM8.

Comme expliqué par téléphone,certains anti-TRPM8 n’ont pas encore été testés chez la souris et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez testerun anticorps chez une espèce ou dans une application qui n'est pas sur la fiche technique, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, un anticorps primaire vous sera offert gratuitement.

Etapes à suivre :

1. Nous confirmer que vous souhaiteriez testerun anticorps Abcam (préciser l'espèce ou l'application)afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de l'anticorps à tester

2. Acheterl'anticorps par téléphone, fax ou internet (www.abcam.com)

3. Le tester

4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
Même si l'anticorps ne pas fonctionne pas dans la nouvelle application ou espèce, après soumission de votre Abreview, vous recevrez quand même votre prochain anticorps gratuitement.

N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

Termes et Conditions : www.abcam.com/collaborationdiscount.

Je vous recontacterai dès que j'aurai reçu les informations sur lesimmunogènes desanti-TRPM8 dont nousavons parlé.

Thank you for your interest in these antibodies. Without a discount the prices range from €360.00 to €370.00. A bulk order discount of approximately 5% is available for orders of 5 units or more of 5 different antibodies. The discount my colleague mentioned in an earlier e-mail is not a discount per se, but an offer of a free antibody in exchange for data from testing of an antibody that has been purchased already. Further, the offer is only available if the data is from a previously untested species or application (which are not covered by our guarantee). For example: Antibody ab74846 has been tested on human samples in several applications: IHC on frozen tissue (IHC-Fr), IHC on paraffin-embedded tissue (IHC-P) and western blotting (WB). Click here (or use the following: https://www.abcam.com/TRPM8-antibody-ab74846.html). If your samples are other than human (e.g., mouse, rat, etc.) or if the application you want to use the antibody for is other than IHC or WB (e.g., immunoprecipitation or ELISA, etc.), our guarantee of a replacement or a credit/refund if the antibody fails does not apply. However, if you send us your data, whether positive or negative, and you have a "discount" code, then the code will be active when we receive your data, and you can use it to receive another antibody for free when you place an order, for instance any of the other TRPM8 antibodies. I will need to know how you intend to use the antibody before I issue the discount code (or codes, if you want to test more than one antibody). I hope this makes sense. Please let me know if you have any questions. I look forward to your reply.