Anti-TRPM8 antibody [EPR4196(2)] (ab109308)


  • Product name
    Anti-TRPM8 antibody [EPR4196(2)]
    See all TRPM8 primary antibodies
  • Description
    Rabbit monoclonal [EPR4196(2)] to TRPM8
  • Host species
  • Tested applications
    Suitable for: ICC, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in the extracellular domain of Human TRPM8.

  • Positive control
    • HT-1376, LnCaP and A549 cell lysates
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/250 - 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa).Can be blocked with TRPM8 peptide (ab187961).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
    • Tissue specificity
      Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
    • Sequence similarities
      Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Long transient receptor potential channel 6 antibody
      • LTrpC-6 antibody
      • LTrpC6 antibody
      • MGC2849 antibody
      • Short form of the TRPM8 cationic channel antibody
      • Transient receptor potential cation channel subfamily M member 8 antibody
      • Transient receptor potential p8 antibody
      • transient receptor potential-p8 antibody
      • Trp p8 antibody
      • Trp-p8 antibody
      • Trpm8 antibody
      • TRPM8_HUMAN antibody
      • TRPP8 antibody
      see all


    • All lanes : Anti-TRPM8 antibody [EPR4196(2)] (ab109308) at 1/1000 dilution

      Lane 1 : HT-1376 cell lysates
      Lane 2 : LnCaP cell lysates
      Lane 3 : A549 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 128 kDa
      Observed band size: 130 kDa (why is the actual band size different from the predicted?)


    This product has been referenced in:
    • Bidaux G  et al. 4TM-TRPM8 channels are new gatekeepers of the ER-mitochondria Ca2+ transfer. Biochim Biophys Acta 1865:981-994 (2018). WB ; Human . Read more (PubMed: 29678654) »
    • Genova T  et al. TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1. J Cell Biol 216:2107-2130 (2017). Read more (PubMed: 28550110) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    The immunogen sequence for this antibody is CVELDEHNLPRFPEW and range is 940-954.

    Thank you for contacting us.

    Yes, we recommend running the WB in reduced and denatured conditions.

    You can find the recommended protocol here:

    I hope this information is helpf...

    Read More

    Thank you for contacting Abcam.

    We spoke earlier about two antibodies:
    anti-BMPR2 ab78422 (
    anti-TRPM8 ab109308 (

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Mouse Tissue sections (Prostate)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Yes - Tween 0.1%
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 1.2% · Temperature: 37°C

    Dr. Gabriel Bidaux

    Verified customer

    Submitted Aug 16 2012

    Merci de votre intérêt pour les anticorps Abcam anti-TRPM8.

    Comme expliqué par téléphone,certains anti-TRPM8 n’ont pas encore été testés chez la souris et nous n’offrons pas d...

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    Thank you for your interest in these antibodies. Without a discount the prices range from €360.00 to €370.00. A bulk order discount of approximately 5% is available for orders of 5 units or more of 5 different antibodies. The discount my collea...

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