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Hello, Under what conditions do you recommend using this Antibody for Western Blotting? Can we follow a standard Denaturing/Reducing protocol, or is this Antibody only tested for whole protein?
Asked on Feb 12 2013
Thank you for contacting us.
Yes, we recommend running the WB in reduced and denatured conditions.
You can find the recommended protocol here: http://www.epitomics.com/products/support/wb
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Feb 12 2013