Overview

  • Product name
    Anti-TRPML1 antibody
  • Description
    Rabbit polyclonal to TRPML1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human TRPML1.

  • Positive control
    • Jurkat Cell Lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Cation channel probably playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. Could play a major role in Ca(2+) transport regulating lysosomal exocytosis.
  • Tissue specificity
    Widely expressed in adult and fetal tissues.
  • Involvement in disease
    Mucolipidosis 4
  • Sequence similarities
    Belongs to the transient receptor (TC 1.A.4) family. Polycystin subfamily. MCOLN1 sub-subfamily.
  • Cellular localization
    Cell membrane. Late endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MCLN1_HUMAN antibody
    • Mcoln 1 antibody
    • Mcoln1 antibody
    • MG 2 antibody
    • MG-2 antibody
    • MG2 antibody
    • ML 4 antibody
    • ML4 antibody
    • MLIV antibody
    • MST080 antibody
    • MSTP080 antibody
    • Mucolipidin antibody
    • Mucolipin 1 antibody
    • Mucolipin-1 antibody
    • Mucolipin1 antibody
    • TRP ML1 antibody
    • TRPML1 antibody
    see all

Images

  • Anti-TRPML1 antibody (ab28508) at 0.5 µg/ml + Jurkat cell lysate

    Predicted band size: 64 kDa

References

This product has been referenced in:
  • Kremer LS  et al. Genetic diagnosis of Mendelian disorders via RNA sequencing. Nat Commun 8:15824 (2017). WB ; Human . Read more (PubMed: 28604674) »
  • Zhang F  et al. TRP-ML1 functions as a lysosomal NAADP-sensitive Ca2+ release channel in coronary arterial myocytes. J Cell Mol Med 13:3174-85 (2009). Read more (PubMed: 18754814) »

See all 2 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (Tris-Glycine 4-15%)
Loading amount
20 µg
Specification
Brain
Blocking step
Licor Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 23 2018

Thank you for your reply.

All of the antibodies suggested have successfully been used in WB with Human samples. They should all work in such application.

All the references we have about our products are listed on the datasheets. Un...

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Thank you for contacting us.

There are seven options available to target Mucolipin 1 with WB; however, depending on the species and the sequence of the peptide used as an immunogen, some of the options may be more convenient than others.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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