EpF-1 and EpF-2 (EpH4 subclones expressing high amounts of Zeb1 and Zeb2 mRNA): According to qPCR data, TRPS1 mRNA levels are decreased in these samples. When compared to housekeeping, TRPS1 protein levels are decreased in these samples in the WB shown.
EpH4 (parental cell line) were TRPS1 is expressed
Figure legend: 1.Ha-Ras-transformed EpH4 cells 2. and 3. EpF-2 and EpF-1 (EpH4 subclones expressing high amounts of Zeb1 and Zeb2 mRNA) 4.EpH4 (parental) 5.EpF-2 6. and 7.EpF-Z1.1 and EpF-Z1.2 (EpF-1 clones transfected with siRNA to knockdown Zeb1) 8. and 9. EpF-Z2.1 and EpF-Z2.2 (EpF-1 clones transfected with siRNA to knockdown Zeb2). Nuclear extracts were prepared in the presence of protein inhibitors and 25ug of protein were run in a 8% gel and transferred to a nitrocellulose membrane (transferred in 1xtransfer buffer+20% MeOH, 1.5h at 110v constant).
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Submitted Jan 28 2014
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