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    trpv1-antibody-ab10295.pdf

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Anti-TRPV1 antibody (ab10295)

  • Datasheet
Reviews (1)Q&A (6)References (5)

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Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody (ab10295)

    Key features and details

    • Guinea pig polyclonal to TRPV1
    • Suitable for: IHC-Fr
    • Reacts with: Rat
    • Isotype: IgG

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    Overview

    • Product name

      Anti-TRPV1 antibody
      See all TRPV1 primary antibodies
    • Description

      Guinea pig polyclonal to TRPV1
    • Host species

      Guinea pig
    • Tested Applications & Species

      Application Species
      IHC-Fr
      Rat
      See all applications and species data
    • Immunogen

      Synthetic peptide within Rat TRPV1 aa 800 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
      Database link: O35433

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituent: Whole serum
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Primary antibody notes

      Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • More Ion Channels
      • Neuroscience
      • Sensory System
      • Somatosensory system
      • Nociception

    Associated products

    • Recombinant Protein

      • Recombinant Human TRPV1 protein (ab159807)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10295 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-Fr
    Rat
    All applications
    Guinea pig
    Application Abreviews Notes
    IHC-Fr (1)
    1/1000.
    Notes
    IHC-Fr
    1/1000.

    Target

    • Function

      Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
    • Tissue specificity

      Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
    • Sequence similarities

      Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
      Contains 6 ANK repeats.
    • Domain

      The association domain (AD) is necessary for self-association.
    • Post-translational
      modifications

      Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
    • Cellular localization

      Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
    • Target information above from: UniProt accession Q8NER1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 100135466 Guinea pig
      • Entrez Gene: 83810 Rat
      • SwissProt: Q6R5A3 Guinea pig
      • SwissProt: O35433 Rat
      • Unigene: 3073 Rat
      • Alternative names

        • Capsaicin receptor antibody
        • DKFZp434K0220 antibody
        • osm 9 like TRP channel 1 antibody
        • Osm-9-like TRP channel 1 antibody
        • OTRPC1 antibody
        • Transient receptor potential cation channel subfamily V member 1 antibody
        • TRPV 1 antibody
        • Trpv1 antibody
        • TRPV1_HUMAN antibody
        • Vanilloid receptor 1 antibody
        • Vanilloid receptor subtype 1 antibody
        • VR 1 antibody
        • VR1 antibody
        see all

      Images

      • Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody (ab10295)
        Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody (ab10295)

        ab10295, at a dilution of 1/1000, staining TRPV1 in rat dorsal root ganglion by Immunohistochemistry (PFA perfusion fixed frozen sections).

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (5)

      Publishing research using ab10295? Please let us know so that we can cite the reference in this datasheet.

      ab10295 has been referenced in 5 publications.

      • Tiede C  et al. Affimer proteins are versatile and renewable affinity reagents. Elife 6:N/A (2017). PubMed: 28654419
      • Marrone MC  et al. TRPV1 channels are critical brain inflammation detectors and neuropathic pain biomarkers in mice. Nat Commun 8:15292 (2017). PubMed: 28489079
      • Charrua A  et al. Co-administration of transient receptor potential vanilloid 4 (TRPV4) and TRPV1 antagonists potentiate the effect of each drug in a rat model of cystitis. BJU Int 115:452-60 (2015). PubMed: 25041193
      • Maione S  et al. Functional interaction between TRPV1 and micro-opioid receptors in the descending antinociceptive pathway activates glutamate transmission and induces analgesia. J Neurophysiol 101:2411-22 (2009). PubMed: 19297510
      • Naeini RS  et al. An N-terminal variant of Trpv1 channel is required for osmosensory transduction. Nat Neurosci 9:93-8 (2006). PubMed: 16327782

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-7 of 7 Abreviews or Q&A

      Question

      Dear Sir/madam
      Do you have the blocking peptide to prove the specificity of Anti-VR1
      antibody (ab10295)?
      The antibody is working well in brain free floating sections, but present
      a widespread labelling. We want to be sure that it is non-specific
      binding.
      Best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Jul 02 2012

      Answer



      I am happy to confirm that the blocking peptide for ab10295 is now available under the product number ab134720:

      https://www.abcam.com/VR1-peptide-ab134720.html (or use the following: https://www.abcam.com/VR1-peptide-ab134720.html).

      I would like to encourage you to submit an Abreview. We and our customer appreciate information about our products very much and you would earn Abpoints that can be redeemed as discount on future orders or Amazon gift vouchers.

      We are looking forward to your order and wish you good luck with your experiments.

      Read More

      Abcam Scientific Support

      Answered on Jul 02 2012

      Question

      Dear xxxx
      First, regarding western blot, it was our intention to test it for the first time and submite a abbreview (no bands were identified in the membrane incubated with the antibody, however the tubulin worked well). I can send you an image, but with absence of signal. Taking in count that the antibody didnt work in IHC, i am not ok to say that it doesnt work in western. I really appreciate if you can replace it with a different lot of the antibody if possible. how can i proceed for that? Do you need an official order?

      With the best regards

      Read More

      Abcam community

      Verified customer

      Asked on May 16 2012

      Answer

      Thank you for your reply.

      I would be happy to arrange for a different lot to be sent to you. You do not need to place a new order, I can arrange it all for you. If you could just confirm that the following address is correct:
      xxxxx


      I look forward to receiving your reply.

      Read More

      Abcam Scientific Support

      Answered on May 16 2012

      Question

      1) Abcam product code ab3002101000 (lot GR64830-N/A)
      2) Abcam order reference number or product batch number- 980870
      3) Description of the problem - Absence of staining
      4) Sample preparation:
      Species: Rat and mouse
      Type of sample: Fresh frozen sections (yes), perfusion fixed frozen sections (yes), PFA/formalin fixed paraffin embedded sections(yes), cells in culture, other:
      Sample preparation
      Positive control: DRG; Spinal Cord
      Negative control: Tissue from VR1- KO mice
      5) Fixation step
      Yes/No - Yes
      If yes: Fixative agent and concentration - PFA; formalin; tamponade formol;
      Fixation time - 2h or overnight for tamponade formol
      Fixation temperature - 4ºC
      6) Antigen retrieval method: Since not used to warming (water bath - 80ºC - 30 min)
      7) Permeabilization method:
      Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers?
      Permeabilizing agent and concentration: Tween - 100
      8) Blocking agent (eg BSA, serum…): NDS
      Concentration - 2% or 10%
      Blocking time- 2h
      Blocking temperature - room temperature
      9) Endogenous peroxidases blocked? Yes
      Endogenous biotins blocked? No
      10) Primary antibody (If more than one was used, describe in “additional notes”) :
      Concentration or dilution: 1: 100; 1:250;1:500;1:1000;1:2000
      Diluent buffer: Blocking solution
      Incubation time 24h, 48h or 72h
      11) Secondary antibody:
      Species:
      Reacts against: anti-guinea pig
      Concentration or dilution: 1:200
      Diluent buffer: Blocking solution
      ncubation time: 2h
      Fluorochrome or enzyme conjugate: biotinilated or alexa fluor 488
      12) Washing after primary and secondary antibodies:
      Buffer - PBST
      Number of washes - 3
      13) Detection method: DAB and fluorescence microscopy
      14) How many times have you run this staining?
      Do you obtain the same results every time?Yes
      What steps have you altered to try and optimize the use of this antibody? Concentration of primary, time and type of fixation; concentration of blocking solution; secondary antibody; time of incubation with primary; antigen retrieval (using or not);
      Document attachment: Attaching images of your IHC is strongly recommended and can greatly speed up our investigation of your problem.
      Well about the choosing of this antibody: it is related with the fact that was already used and validated in studies of my area of interest - RVM in a papper referenced in your site.
      With the best regards

      Read More

      Abcam community

      Verified customer

      Asked on May 16 2012

      Answer

      Thank you very much for providing that extra information. It has helped greatly in understanding the problems encountered and the optimisation you have already performed.

      The anti-VR1 antibody (ab10295) has only been validated in frozen sections with rat tissue. This does not mean that it will not work in order tissue preparations or species but we do not currently have any information to support this. It may be that although the immunogen used to raise the antibody,dispite sharing 95% homology with the mouse protein, is not able to detect this species. Additionally, as paraffin embedded sections have not been used this may not be ideal with this antibody, as is the case with the Western blotting too. May I ask, what was the result of the Western blot? Could you send me an image of the blot?

      We would expect the antibody to be able to detect the protein in your rat tissue with frozen sections, which you have not observed. I am contacting the source of this antibody to find out the exact protocol used to prepare the tissue in order to see if this could be contributing to the lack of signal.

      In the meantime, if you would like, I can offer you a replacement vial of a different lot or a different VR1 antibody altogether such as rabbit polyclonal ab31895. This has been used in both mouse and rat tissue with paraffin embedded and frozen sections.

      I look forward to receiving your reply.

      Read More

      Abcam Scientific Support

      Answered on May 16 2012

      Question

      Product code: 10295
      Lot number: GR64830-1
      Inquiry: Good afternoon I am writing to your company to make you know that the lot of antibody we received doesnt work neither in IHC of DRG, SC or RVM sections. We tested different protocols of fixation, cuts thickness, different secondary but with no positive results. We also tested it in western blotting, but with no positive results. I would like to know if the guarantee covers a reposition of a new lot of antibody and how i can procceed for it. Our requisition was made in november (order number 980870), since then we are trying to put it working but without success, which makes us to believe that the problem resides in the primary. With the best regards

      Read More

      Abcam community

      Verified customer

      Asked on May 15 2012

      Answer

      Thank you for contacting us and reporting the problems you have encountered in using the anti-VR1 antibody (ab10295). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

      I appreciate the time you have spent in the lab trying to optimise the results with this antibody. However, in order to more fully understand what may be contributing to the problems I would really appreciate it if you could give me a few more details on how the antibody has been used. To this end I have attached a questionnaire to this email. OnceI receive the completed questionnaire,I will look at the protocol and see if there are any suggestionsI can make that may improve the results.

      This information will also allow us to investigate this case internally and initiate anyadditional testing where necessary. Whilst the product was purchased outside of the last 6 months and is therefore not covered by the Abpromise guarantee, I understand your concerns and the effort you have made and I would therefore be willing to extend this cover if the antibody has been usedaccording to our Abpromise, andwould be happy to replace or refund the antibody.

      May I ask,is there a specificreason whyab10295(guinea pig polyclonal) was chosen and not one of our other antibodies againstVR1?

      I look forward to receiving your reply.

      Read More

      Abcam Scientific Support

      Answered on May 15 2012

      Question

      Dear Sir/Madam we are interested to test ab10295 in western blot. Please send me the discount code to proceed with the order. We will submit an abreview as soon as possible

      Read More

      Abcam community

      Verified customer

      Asked on Nov 04 2011

      Answer

      DISCOUNT CODE: ****** Expiration date: ***** I am very pleased to hear you would like to accept our offer and test ab10295 in western blot. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for western blot and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

      Read More

      Abcam Scientific Support

      Answered on Nov 04 2011

      Question

      We would like to use the Anti-VR1 antibody (ab10295)in our research. However no data are available if this antibody is suitable for WB or not. We want to buy an TRPV1 antibody made in guinea pig that could be uses in both appications. Could you please send me any information or a sample to me to test it in WB?

      Read More

      Abcam community

      Verified customer

      Asked on Nov 04 2011

      Answer

      Thank you for contacting Abcam about ab10295. We do not sell sample sizes of our antibodies but since ab10295  has not been tested in western blot, I can offer a discount off a future purchase if you buy ab10295 now, test it in western blot and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab10295 in western blotI will then send a discount code. This code must be issued before purchasing ab10295 so please wait for my reply before ordering. 2. Purchase ab10295 either by phone, fax, or online (www.abcam.com). 3. Test it in western blot. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab10295 turns out to be unsuitable for western blot you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

      Read More

      Abcam Scientific Support

      Answered on Nov 04 2011

      Immunohistochemistry (Frozen sections) abreview for Anti-TRPV1 antibody

      Good
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rat Tissue sections (brain)
      Specification
      brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 13 2008

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