Anti-TRPV1 antibody (ab10295)
Key features and details
- Guinea pig polyclonal to TRPV1
- Suitable for: IHC-Fr
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-TRPV1 antibody
See all TRPV1 primary antibodies -
Description
Guinea pig polyclonal to TRPV1 -
Host species
Guinea pig -
Tested Applications & Species
Application Species IHC-Fr Rat -
Immunogen
Synthetic peptide within Rat TRPV1 aa 800 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O35433 -
General notes
Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Primary antibody notes
Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-Fr |
Rat
|
All applications |
Guinea pig
|
Application | Abreviews | Notes |
---|---|---|
IHC-Fr | (1) |
1/1000.
|
Notes |
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IHC-Fr
1/1000. |
Target
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Function
Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis. -
Tissue specificity
Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level). -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
Contains 6 ANK repeats. -
Domain
The association domain (AD) is necessary for self-association. -
Post-translational
modificationsPhosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane. - Information by UniProt
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Database links
- Entrez Gene: 100135466 Guinea pig
- Entrez Gene: 83810 Rat
- SwissProt: Q6R5A3 Guinea pig
- SwissProt: O35433 Rat
- Unigene: 3073 Rat
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Alternative names
- Capsaicin receptor antibody
- DKFZp434K0220 antibody
- osm 9 like TRP channel 1 antibody
see all
Images
Datasheets and documents
References (5)
ab10295 has been referenced in 5 publications.
- Tiede C et al. Affimer proteins are versatile and renewable affinity reagents. Elife 6:N/A (2017). PubMed: 28654419
- Marrone MC et al. TRPV1 channels are critical brain inflammation detectors and neuropathic pain biomarkers in mice. Nat Commun 8:15292 (2017). PubMed: 28489079
- Charrua A et al. Co-administration of transient receptor potential vanilloid 4 (TRPV4) and TRPV1 antagonists potentiate the effect of each drug in a rat model of cystitis. BJU Int 115:452-60 (2015). PubMed: 25041193
- Maione S et al. Functional interaction between TRPV1 and micro-opioid receptors in the descending antinociceptive pathway activates glutamate transmission and induces analgesia. J Neurophysiol 101:2411-22 (2009). PubMed: 19297510
- Naeini RS et al. An N-terminal variant of Trpv1 channel is required for osmosensory transduction. Nat Neurosci 9:93-8 (2006). PubMed: 16327782