Anti-TRPV1 antibody (ab10295)

I am happy to confirm that the blocking peptide for ab10295 is now available under the product number ab134720:

https://www.abcam.com/VR1-peptide-ab134720.html (or use the following: https://www.abcam.com/VR1-peptide-ab134720.html).

I would like to encourage you to submit an Abreview. We and our customer appreciate information about our products very much and you would earn Abpoints that can be redeemed as discount on future orders or Amazon gift vouchers.

We are looking forward to your order and wish you good luck with your experiments.

Thank you for your reply.

I would be happy to arrange for a different lot to be sent to you. You do not need to place a new order, I can arrange it all for you. If you could just confirm that the following address is correct:
xxxxx


I look forward to receiving your reply.

Thank you very much for providing that extra information. It has helped greatly in understanding the problems encountered and the optimisation you have already performed.

The anti-VR1 antibody (ab10295) has only been validated in frozen sections with rat tissue. This does not mean that it will not work in order tissue preparations or species but we do not currently have any information to support this. It may be that although the immunogen used to raise the antibody,dispite sharing 95% homology with the mouse protein, is not able to detect this species. Additionally, as paraffin embedded sections have not been used this may not be ideal with this antibody, as is the case with the Western blotting too. May I ask, what was the result of the Western blot? Could you send me an image of the blot?

We would expect the antibody to be able to detect the protein in your rat tissue with frozen sections, which you have not observed. I am contacting the source of this antibody to find out the exact protocol used to prepare the tissue in order to see if this could be contributing to the lack of signal.

In the meantime, if you would like, I can offer you a replacement vial of a different lot or a different VR1 antibody altogether such as rabbit polyclonal ab31895. This has been used in both mouse and rat tissue with paraffin embedded and frozen sections.

I look forward to receiving your reply.

Thank you for contacting us and reporting the problems you have encountered in using the anti-VR1 antibody (ab10295). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I appreciate the time you have spent in the lab trying to optimise the results with this antibody. However, in order to more fully understand what may be contributing to the problems I would really appreciate it if you could give me a few more details on how the antibody has been used. To this end I have attached a questionnaire to this email. OnceI receive the completed questionnaire,I will look at the protocol and see if there are any suggestionsI can make that may improve the results.

This information will also allow us to investigate this case internally and initiate anyadditional testing where necessary. Whilst the product was purchased outside of the last 6 months and is therefore not covered by the Abpromise guarantee, I understand your concerns and the effort you have made and I would therefore be willing to extend this cover if the antibody has been usedaccording to our Abpromise, andwould be happy to replace or refund the antibody.

May I ask,is there a specificreason whyab10295(guinea pig polyclonal) was chosen and not one of our other antibodies againstVR1?

I look forward to receiving your reply.

DISCOUNT CODE: ****** Expiration date: ***** I am very pleased to hear you would like to accept our offer and test ab10295 in western blot. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for western blot and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

Thank you for contacting Abcam about ab10295. We do not sell sample sizes of our antibodies but since ab10295  has not been tested in western blot, I can offer a discount off a future purchase if you buy ab10295 now, test it in western blot and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab10295 in western blotI will then send a discount code. This code must be issued before purchasing ab10295 so please wait for my reply before ordering. 2. Purchase ab10295 either by phone, fax, or online (www.abcam.com). 3. Test it in western blot. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab10295 turns out to be unsuitable for western blot you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.