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Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)

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Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
  • Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
  • Flow Cytometry (Intracellular) - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
  • Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
  • Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)

Key features and details

  • Mouse monoclonal [BS397] to TRPV1 - C-terminal
  • Suitable for: Flow Cyt (Intra), WB, IHC-Fr, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG2b

Overview

  • Product name

    Anti-TRPV1 antibody [BS397] - C-terminal
    See all TRPV1 primary antibodies

  • Description

    Mouse monoclonal [BS397] to TRPV1 - C-terminal

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Fr, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Guinea pig

  • Immunogen

    Synthetic peptide within Rat TRPV1 aa 800 to the C-terminus (C terminal) (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: O35433

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: PC-12 cell lysates; Mouse brain homogenate; forskolin and NGF stimulated 50B11 hybrid mouse and rat DRG cell lysates; NGF-stimulated PC-12 cell lysates. IHC-Fr: Mouse dorsal root ganglia. ICC/IF: C6 cells. Flow Cyt (Intra): PC-12 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use 2µg for 106 cells.
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 95 kDa.
IHC-Fr
Use a concentration of 1 - 10 µg/ml.

Pre-absorption with immunogen obliterates positive staining. Frozen or PEG embedded tissues tested.
ICC/IF
Use a concentration of 1 - 2 µg/ml.

Requires permeabilization step due to antigen epitope is intracellular
Notes
Flow Cyt (Intra)
Use 2µg for 106 cells.
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 95 kDa.
IHC-Fr
Use a concentration of 1 - 10 µg/ml.

Pre-absorption with immunogen obliterates positive staining. Frozen or PEG embedded tissues tested.
ICC/IF
Use a concentration of 1 - 2 µg/ml.

Requires permeabilization step due to antigen epitope is intracellular

Target

  • Function

    Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.

  • Tissue specificity

    Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).

  • Sequence similarities

    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.

  • Domain

    The association domain (AD) is necessary for self-association.

  • Post-translational
    modifications

    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.

  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Capsaicin receptor antibody
      • DKFZp434K0220 antibody
      • osm 9 like TRP channel 1 antibody
      • Osm-9-like TRP channel 1 antibody
      • OTRPC1 antibody
      • Transient receptor potential cation channel subfamily V member 1 antibody
      • TRPV 1 antibody
      • Trpv1 antibody
      • TRPV1_HUMAN antibody
      • Vanilloid receptor 1 antibody
      • Vanilloid receptor subtype 1 antibody
      • VR 1 antibody
      • VR1 antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
      Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)

      Immunohistochemical analysis of mouse dorsal root ganglia labelling TRPV1 with ab203103 at 10 µg/mL. Visualized with anti-mouse-Cy3 conjugate (red).

       

    • Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
      Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
      Lane 1 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml (overnight)
      Lane 2 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml ((pre-absorbed))

      All lanes : Rat PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate

      Lysates/proteins at 80 µg per lane.

      Secondary
      All lanes : Anti-mouse-HRP at 1/6000 dilution

      Developed using the ECL technique.

      Predicted band size: 95 kDa



      SDS-PAGE: denatured and reduced

    • Immunocytochemistry/ Immunofluorescence - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
      Immunocytochemistry/ Immunofluorescence - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)

      ab203103 staining TRPV1 in C6 (Rat glial tumor cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

      Cells were fixed with 4% formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% horse serum. Samples were incubated with primary antibody (2 μg/ml, panel B) for 1 hour. A CF488A-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody (5 μg/ml). Nuclei were stained with Hoechst dye (panel A) and panel C shows the merged image.

    • Flow Cytometry (Intracellular) - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
      Flow Cytometry (Intracellular) - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)

      Analysis of TRPV1 expression in PC-12 (Rat adrenal gland pheochromocytoma cell line) cell line by Flow Cyt (Intra) (black solid).

      Cells were fixed and permeabilized in absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, then blocked with 1% BSA. Incubated with primary antibody for 30 minutes at RT. Secondary antibody used is a Goat anti-mouse PE labeled secondary antibody (1:100 dilution), 20 minutes in dark at room temperature.

      Negative control: Non-specific Control IgG, clone X63 (black dashed).

    • Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
      Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
      All lanes : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 1 µg/ml

      Lane 1 : Control
      Lane 2 : Forskolin stimulated 50B11 hybrid mouse x rat DRG cell lysate
      Lane 3 : Forskolin and NGF stimulated 50B11 hybrid mouse x rat DRG cell lysate
      Lane 4 : NGF-stimulated PC-12 (Rat glial tumor cell line) cell lysate (NGF control)

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 95 kDa



      (denatured and reduced)

    • Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
      Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
      Lane 1 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml
      Lane 2 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 1 µg/ml
      Lane 3 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml (pre-absorbed)

      All lanes : Mouse brain homogenate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Anti-mouse-HRP at 1/6000 dilution

      Developed using the ECL technique.

      Predicted band size: 95 kDa



      SDS-PAGE: denatured and reduced. ab203103 detects bands at 90-100 kDa and 180-200 kDa corresponding to TRPV1 monomer and dimer.

    References (12)

    Publishing research using ab203103? Please let us know so that we can cite the reference in this datasheet.

    ab203103 has been referenced in 12 publications.

    • Gao X  et al. Cross-effect of TRPV1 and EP3 receptor on coughs and bronchopulmonary C-neural activities. PLoS One 16:e0246375 (2021). PubMed: 33529249
    • Zhang Y  et al. Therapeutic effects of peripherally administrated neural crest stem cells on pain and spinal cord changes after sciatic nerve transection. Stem Cell Res Ther 12:180 (2021). PubMed: 33722287
    • Caires R  et al. Deficiency of Inositol Monophosphatase Activity Decreases Phosphoinositide Lipids and Enhances TRPV1 Function In Vivo. J Neurosci 41:408-423 (2021). PubMed: 33239401
    • Galiazzo G  et al. Cellular distribution of cannabinoid-related receptors TRPV1, PPAR-gamma, GPR55 and GPR3 in the equine cervical dorsal root ganglia. Equine Vet J N/A:N/A (2021). PubMed: 34418142
    • O'Leary C  et al. Involvement of TRPV1 and TRPV4 Channels in Retinal Angiogenesis. Invest Ophthalmol Vis Sci 60:3297-3309 (2019). PubMed: 31369032
    View all Publications for this product

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