Key features and details
- Goat polyclonal to TRPV3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRPV3 antibody
See all TRPV3 primary antibodies
DescriptionGoat polyclonal to TRPV3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- WB: HEK-293 lysate overexpressing Human TRPV3 with DYKDDDDK tag.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab240452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 91 kDa).
A 1 hour primary incubation is recommended for this product.
FunctionPutative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity.
Tissue specificityAbundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level).
Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV3 sub-subfamily.
Contains 3 ANK repeats.
- Information by UniProt
- 1110036I10Rik antibody
- MGC124324 antibody
- MGC124325 antibody
Lane 1 : Anti-TRPV3 antibody (ab240452) at 0.5 µg/ml
Lane 2 : Anti-TRPV3 antibody (ab240452) at 1 mg/ml
Lane 3 : anti- DYKDDDDK Tag at 1/10000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) lysate (in RIPA buffer) overexpressing Human TRPV3 with DYKDDDDK tag
Lane 2 : Mock-transfected HEK-293 lysate
Lane 3 : HEK-293 lysate (in RIPA buffer) overexpressing Human TRPV3 with DYKDDDDK tag
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 91 kDa
Primary incubations were for 1 hour.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240452 has not yet been referenced specifically in any publications.