• Product name

    Anti-TRPV3 antibody [N15/4]
    See all TRPV3 primary antibodies
  • Description

    Mouse monoclonal [N15/4] to TRPV3
  • Host species

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Rat TRPV3 aa 774-791. From the cytoplasmic C-terminus


    Database link: NP_001020928

  • Positive control

    • COS-1 cell lysate transiently transfected with TRPV3.
  • General notes

    The clone number has been updated from S15-4 to N15/4, both clone numbers name the same antibody clone.




Our Abpromise guarantee covers the use of ab85022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa.
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.
IHC-P 1/1000.


  • Function

    Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity.
  • Tissue specificity

    Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level).
  • Sequence similarities

    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV3 sub-subfamily.
    Contains 3 ANK repeats.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • 1110036I10Rik antibody
    • MGC124324 antibody
    • MGC124325 antibody
    • Transient receptor potential cation channel subfamily V member 3 antibody
    • TrpV3 antibody
    • Trpv3 heat sensitive channel antibody
    • TRPV3_HUMAN antibody
    • Vanilloid receptor 3 antibody
    • Vanilloid receptor like 3 antibody
    • Vanilloid receptor-like 3 antibody
    • vanilloid receptor-related osmotically activated channel protein antibody
    • VRL 3 antibody
    • VRL-3 antibody
    • VRL3 antibody
    see all


  • ab85022 staining TRPV3 in mouse back skin tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.

  • Overlay histogram showing SH-SY5Y cells stained with ab85022 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85022, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • All lanes : Anti-TRPV3 antibody [N15/4] (ab85022) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from mixture of human cell lines A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G at 10 µg

    Predicted band size: 90 kDa

  • ab85022 staining TRPV3 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.


This product has been referenced in:

  • Sakakibara A  et al. Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking. PLoS One 12:e0169723 (2017). Read more (PubMed: 28081185) »
  • Pires PW  et al. Unitary TRPV3 channel Ca2+ influx events elicit endothelium-dependent dilation of cerebral parenchymal arterioles. Am J Physiol Heart Circ Physiol 309:H2031-41 (2015). Read more (PubMed: 26453324) »
See all 3 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate 10 mM, pH 6.0
Human Tissue sections (Skin)

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Submitted Dec 13 2013

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