Key features and details
- Mouse monoclonal [N15/4] to TRPV3
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Product nameAnti-TRPV3 antibody [N15/4]
See all TRPV3 primary antibodies
DescriptionMouse monoclonal [N15/4] to TRPV3
Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- COS-1 cell lysate transiently transfected with TRPV3.
The clone number has been updated from S15-4 to N15/4, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab85022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa.|
|Flow Cyt||Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionPutative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity.
Tissue specificityAbundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level).
Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV3 sub-subfamily.
Contains 3 ANK repeats.
- Information by UniProt
- 1110036I10Rik antibody
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ab85022 staining TRPV3 in mouse back skin tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.
Overlay histogram showing SH-SY5Y cells stained with ab85022 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85022, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
All lanes : Anti-TRPV3 antibody [N15/4] (ab85022) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from mixture of human cell lines A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G at 10 µg
Predicted band size: 90 kDa
ab85022 staining TRPV3 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab85022 has been referenced in 3 publications.
- Sakakibara A et al. Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking. PLoS One 12:e0169723 (2017). PubMed: 28081185
- Pires PW et al. Unitary TRPV3 channel Ca2+ influx events elicit endothelium-dependent dilation of cerebral parenchymal arterioles. Am J Physiol Heart Circ Physiol 309:H2031-41 (2015). PubMed: 26453324
- Albert ES et al. TRPV4 channels mediate the infrared laser-evoked response in sensory neurons. J Neurophysiol 107:3227-34 (2012). PubMed: 22442563