Key features and details
- Rabbit polyclonal to TRPV4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRPV4 antibody
See all TRPV4 primary antibodies
DescriptionRabbit polyclonal to TRPV4
Specificityab191580 is predicted to not cross-react with other TRP protein family members.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Horse
Synthetic peptide corresponding to Human TRPV4 (internal sequence). (18 amino acids). NP_067638.3.
Database link: Q9HBA0
- Human testis tissue lysate; Human lung tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 98 kDa).|
|IHC-P||Use a concentration of 2 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionNon-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.
Tissue specificityFound in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
Involvement in diseaseBrachyolmia 3
Spondylometaphyseal dysplasia Kozlowski type
Distal spinal muscular atrophy, congenital non-progressive
Charcot-Marie-Tooth disease 2C
Scapuloperoneal spinal muscular atrophy
Spondyloepiphyseal dysplasia Maroteaux type
Digital arthropathy-brachydactyly, familial
Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
Contains 3 ANK repeats.
modificationsPhosphorylation results in enhancement of its channel function.
Cellular localizationCell membrane and Cell membrane. Cell junction > adherens junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
- Information by UniProt
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Western Blot of human testis tissue lysate, SK-N-SH cell lysate labeling TRPV4 with Anti-TRPV4 antibody (ab191580) at 1µg/ml.
Immunofluorescent analysis of TRPV4 expression in human kidney tissue with ab191580 at 5µg/ml.
Anti-TRPV4 antibody (ab191580) at 1 µg/ml + Human testis tissue lysate at 15 µg
Predicted band size: 98 kDa
Immunohistochemical analysis of paraffin-embedded human lung tissue labeling TRPV4 with ab191580 at 5 µg/ml.
Immunofluorescent analysis of human lung tissue labeling TRPV4 with ab191580 at 20 µg/ml.
ab191580 has been referenced in 1 publication.
- Nam S et al. Cell cycle progression in confining microenvironments is regulated by a growth-responsive TRPV4-PI3K/Akt-p27Kip1 signaling axis. Sci Adv 5:eaaw6171 (2019). PubMed: 31457089