Anti-TRPV4 antibody (ab219192)
Key features and details
- Rabbit polyclonal to TRPV4
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRPV4 antibody
See all TRPV4 primary antibodies -
Description
Rabbit polyclonal to TRPV4 -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other human proteins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Pig, Monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Human TRPV4 (internal sequence). (20 amino acid peptide).
Database link: Q9HBA0 -
Positive control
- Human pancreas (islet) tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.099% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. -
Tissue specificity
Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level). -
Involvement in disease
Brachyolmia 3
Spondylometaphyseal dysplasia Kozlowski type
Metatropic dysplasia
Distal spinal muscular atrophy, congenital non-progressive
Charcot-Marie-Tooth disease 2C
Scapuloperoneal spinal muscular atrophy
Spondyloepiphyseal dysplasia Maroteaux type
Parastremmatic dwarfism
Digital arthropathy-brachydactyly, familial -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
Contains 3 ANK repeats. -
Post-translational
modificationsPhosphorylation results in enhancement of its channel function. -
Cellular localization
Cell membrane and Cell membrane. Cell junction > adherens junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 395427 Chicken
- Entrez Gene: 540259 Cow
- Entrez Gene: 486308 Dog
- Entrez Gene: 101136579 Gorilla
- Entrez Gene: 101826031 Hamster
- Entrez Gene: 100066552 Horse
- Entrez Gene: 59341 Human
- Entrez Gene: 63873 Mouse
see all -
Alternative names
- BCYM3 antibody
- CMT2C antibody
- HMSN2C antibody
see all
Images
Datasheets and documents
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Datasheet download
References (1)
ab219192 has been referenced in 1 publication.
- Ackermann K et al. Expression Profiles of ASIC1/2 and TRPV1/4 in Common Skin Tumors. Int J Mol Sci 22:N/A (2021). PubMed: 34199609