Product nameAnti-TRPV4 antibody
See all TRPV4 primary antibodies
DescriptionRabbit polyclonal to TRPV4
Tested applicationsSuitable for: ICC/IF, IHC-FoFr, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Horse
- This antibody gave a positive signal in Mouse Brain tissue lysate. This antibody gave a positive signal in Mouse Brain FFPE tissue.
Although some of our customers have had good results in Human and Rat (89% homology with the immunogen), we do not batch test this antibody in these species. Due to the polyclonal nature of this antibody, some batches many not work in Human or Rat.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab39260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 98 kDa).|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionNon-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.
Tissue specificityFound in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
Involvement in diseaseBrachyolmia 3
Spondylometaphyseal dysplasia Kozlowski type
Distal spinal muscular atrophy, congenital non-progressive
Charcot-Marie-Tooth disease 2C
Scapuloperoneal spinal muscular atrophy
Spondyloepiphyseal dysplasia Maroteaux type
Digital arthropathy-brachydactyly, familial
Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
Contains 3 ANK repeats.
modificationsPhosphorylation results in enhancement of its channel function.
Cellular localizationCell membrane and Cell membrane. Cell junction > adherens junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
- Information by UniProt
- BCYM3 antibody
- CMT2C antibody
- HMSN2C antibody
IHC image of ab39260 staining in mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The section was then incubated with ab39260, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Anti-TRPV4 antibody (ab39260) at 2 µg/ml + Brain (Mouse) Tissue Lysate at 25 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 98 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
ab39260 staining of TRPV4 -/- (upper panel) and wildtype (lower panel) adult mouse kidney tissue sections, showing good immunostaining in the wildtype tissue and no immunostaining in the TRPV4 -/- tissue. Formalin/PFA-fixed paraffin-embedded sections of mouse kidney tissue were incubated with ab39260 (1/200) for 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0. A biotin-conjugated goat anti-rabbit antibody was used as the secondary.
ICC/IF image of ab39260 stained PC12 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab39260, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
IHC-FoFr image of TRPV4 staining on Rat DRG sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat. The image shows DRG neurons (left), nerve taken at the level of the root (right)
All lanes : Anti-TRPV4 antibody (ab39260) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from MEF cells
Lane 2 : Whole cell lysate prepared from F9 cells
Lane 3 : Whole cell lysate prepared from GC-1 cells
Lane 4 : Whole cell lysate prepared from NIH-3T3 cells
Lysates/proteins at 20 µg per lane.
All lanes : HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 98 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
This antibody detects a band of ~125 kDa in 4 different mouse cell lines. This is lower than the predicted molecular weight of TRPV4 on SDS-PAGE (100 kDa). However, there are published reports of TRPV4 running at >120 kDa PMID: 16368742 PMID: 12538589, possibly as a result of glycosylation.
This product has been referenced in:
- Minami A et al. Hyperbaric oxygen significantly improves frequent urination, hyperalgesia, and tissue damage in a mouse long-lasting cystitis model induced by an intravesical instillation of hydrogen peroxide. Neurourol Urodyn 38:97-106 (2019). Read more (PubMed: 30411813) »
- Lee HP et al. Volume expansion and TRPV4 activation regulate stem cell fate in three-dimensional microenvironments. Nat Commun 10:529 (2019). Read more (PubMed: 30705265) »