• Product name

  • Description

    Rabbit polyclonal to TRPV4
  • Host species

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 720-769 (


    ) of Human TRPV4 isoform 2 (NP_671737)

  • Positive control

    • HT1080 cell lysate



Our Abpromise guarantee covers the use of ab94868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function

    Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.
  • Tissue specificity

    Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
  • Involvement in disease

    Brachyolmia 3
    Spondylometaphyseal dysplasia Kozlowski type
    Metatropic dysplasia
    Distal spinal muscular atrophy, congenital non-progressive
    Charcot-Marie-Tooth disease 2C
    Scapuloperoneal spinal muscular atrophy
    Spondyloepiphyseal dysplasia Maroteaux type
    Parastremmatic dwarfism
    Digital arthropathy-brachydactyly, familial
  • Sequence similarities

    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
    Contains 3 ANK repeats.
  • Post-translational

    Phosphorylation results in enhancement of its channel function.
  • Cellular localization

    Cell membrane and Cell membrane. Cell junction > adherens junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • BCYM3 antibody
    • CMT2C antibody
    • HMSN2C antibody
    • osm 9 like TRP channel 4 antibody
    • Osm-9-like TRP channel 4 antibody
    • OSM9 like transient receptor potential channel 4 antibody
    • Osmosensitive transient receptor potential channel 4 antibody
    • OTRPC 4 antibody
    • OTRPC4 antibody
    • SMAL antibody
    • SPSMA antibody
    • SSQTL1 antibody
    • Transient receptor potential cation channel subfamily V member 4 antibody
    • Transient receptor potential protein 12 antibody
    • TRP 12 antibody
    • TRP12 antibody
    • TRPV 4 antibody
    • TrpV4 antibody
    • TRPV4_HUMAN antibody
    • Vanilloid receptor like channel 2 antibody
    • Vanilloid receptor like protein 2 antibody
    • Vanilloid receptor related osmotically activated channel antibody
    • Vanilloid receptor-like channel 2 antibody
    • Vanilloid receptor-like protein 2 antibody
    • Vanilloid receptor-related osmotically-activated channel antibody
    • VR 4 antibody
    • VR OAC antibody
    • VR-OAC antibody
    • VR4 antibody
    • VRL 2 antibody
    • VRL-2 antibody
    • VRL2 antibody
    • VROAC antibody
    see all


  • Western blot analysis of PANC1 cells labeling TRPV4 with ab94868 at 1.0µg/ml.
  • ab94868 staining TRPV4 in cultured hippocampal slices of rat brain by Immunohistochemistry - PFA fixed sections.

    Slice cultures attached to the PTFE membrane were fixed with 4% paraformaldehyde in PBS for 20 minutes at room temperature. They were permeabilized with 1% Triton X-100 in PBS overnight at 4 °C, then blocked with 3% bovine serum albumin (BSA) in PBS and incubated overnight at 4 °C with ab94868 (Red) at a 1/500 dilution. Following washes with PBS and blocking with 3% BSA, tissue was incubated for 1 hour at room temperature with Alexa Fluor 568-conjugated goat anti-rabbit antibody diluted 1/500 in blocking solution. Tissue was washed in PBS, mounted with DAPI.

    Blue: DAPI.
    Green: anti-Map2.
    Red: ab94868.
  • Anti-TRPV4 antibody (ab94868) at 1 µg/ml + HT1080 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 91 kDa

    Gel concentration: 6-18%


This product has been referenced in:

  • Fang Y  et al. Pharmacological inhibition of TRPV4 channel suppresses malignant biological behavior of hepatocellular carcinoma via modulation of ERK signaling pathway. Biomed Pharmacother 101:910-919 (2018). WB . Read more (PubMed: 29635900) »
  • Shenton FC & Pyner S Transient receptor potential vanilloid type 4 is expressed in vasopressinergic neurons within the magnocellular subdivision of the rat paraventricular nucleus of the hypothalamus. J Comp Neurol 526:3035-3044 (2018). Read more (PubMed: 30078222) »
See all 6 Publications for this product

Customer reviews and Q&As

Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (Bio-Rad 4–20% Mini-PROTEAN­ TGX™ Gel, 12 well, 20 ´l 456-1095)
Pig Tissue lysate - whole (coronary artery)
coronary artery
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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Submitted Aug 29 2014

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