Product nameAnti-TRPV4 antibody
See all TRPV4 primary antibodies
DescriptionRabbit polyclonal to TRPV4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
- HT1080 cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab94868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionNon-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.
Tissue specificityFound in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
Involvement in diseaseBrachyolmia 3
Spondylometaphyseal dysplasia Kozlowski type
Distal spinal muscular atrophy, congenital non-progressive
Charcot-Marie-Tooth disease 2C
Scapuloperoneal spinal muscular atrophy
Spondyloepiphyseal dysplasia Maroteaux type
Digital arthropathy-brachydactyly, familial
Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
Contains 3 ANK repeats.
modificationsPhosphorylation results in enhancement of its channel function.
Cellular localizationCell membrane and Cell membrane. Cell junction > adherens junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
- Information by UniProt
- BCYM3 antibody
- CMT2C antibody
- HMSN2C antibody
Western blot analysis of PANC1 cells labeling TRPV4 with ab94868 at 1.0µg/ml.
ab94868 staining TRPV4 in cultured hippocampal slices of rat brain by Immunohistochemistry - PFA fixed sections.
Slice cultures attached to the PTFE membrane were fixed with 4% paraformaldehyde in PBS for 20 minutes at room temperature. They were permeabilized with 1% Triton X-100 in PBS overnight at 4 °C, then blocked with 3% bovine serum albumin (BSA) in PBS and incubated overnight at 4 °C with ab94868 (Red) at a 1/500 dilution. Following washes with PBS and blocking with 3% BSA, tissue was incubated for 1 hour at room temperature with Alexa Fluor 568-conjugated goat anti-rabbit antibody diluted 1/500 in blocking solution. Tissue was washed in PBS, mounted with DAPI.Blue: DAPI.
Green: anti-Map2.Red: ab94868.
Anti-TRPV4 antibody (ab94868) at 1 µg/ml + HT1080 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 91 kDa
Gel concentration: 6-18%
This product has been referenced in:
- Fang Y et al. Pharmacological inhibition of TRPV4 channel suppresses malignant biological behavior of hepatocellular carcinoma via modulation of ERK signaling pathway. Biomed Pharmacother 101:910-919 (2018). WB . Read more (PubMed: 29635900) »
- Shenton FC & Pyner S Transient receptor potential vanilloid type 4 is expressed in vasopressinergic neurons within the magnocellular subdivision of the rat paraventricular nucleus of the hypothalamus. J Comp Neurol 526:3035-3044 (2018). Read more (PubMed: 30078222) »