Overview

  • Product name

    Anti-TRPV4 antibody [EPR22622-54]
    See all TRPV4 primary antibodies
  • Description

    Rabbit monoclonal [EPR22622-54] to TRPV4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IP or WB
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse TRPV4 aa 850 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9EPK8

  • Positive control

    • IHC-P: Mouse cerebrum and kidney tissue. Rat kidney and spleen tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab231772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IP or WB.
  • Target

    • Function

      Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.
    • Tissue specificity

      Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
    • Involvement in disease

      Brachyolmia 3
      Spondylometaphyseal dysplasia Kozlowski type
      Metatropic dysplasia
      Distal spinal muscular atrophy, congenital non-progressive
      Charcot-Marie-Tooth disease 2C
      Scapuloperoneal spinal muscular atrophy
      Spondyloepiphyseal dysplasia Maroteaux type
      Parastremmatic dwarfism
      Digital arthropathy-brachydactyly, familial
    • Sequence similarities

      Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
      Contains 3 ANK repeats.
    • Post-translational
      modifications

      Phosphorylation results in enhancement of its channel function.
    • Cellular localization

      Cell membrane and Cell membrane. Cell junction > adherens junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
    • Information by UniProt
    • Database links

    • Alternative names

      • BCYM3 antibody
      • CMT2C antibody
      • HMSN2C antibody
      • osm 9 like TRP channel 4 antibody
      • Osm-9-like TRP channel 4 antibody
      • OSM9 like transient receptor potential channel 4 antibody
      • Osmosensitive transient receptor potential channel 4 antibody
      • OTRPC 4 antibody
      • OTRPC4 antibody
      • SMAL antibody
      • SPSMA antibody
      • SSQTL1 antibody
      • Transient receptor potential cation channel subfamily V member 4 antibody
      • Transient receptor potential protein 12 antibody
      • TRP 12 antibody
      • TRP12 antibody
      • TRPV 4 antibody
      • TrpV4 antibody
      • TRPV4_HUMAN antibody
      • Vanilloid receptor like channel 2 antibody
      • Vanilloid receptor like protein 2 antibody
      • Vanilloid receptor related osmotically activated channel antibody
      • Vanilloid receptor-like channel 2 antibody
      • Vanilloid receptor-like protein 2 antibody
      • Vanilloid receptor-related osmotically-activated channel antibody
      • VR 4 antibody
      • VR OAC antibody
      • VR-OAC antibody
      • VR4 antibody
      • VRL 2 antibody
      • VRL-2 antibody
      • VRL2 antibody
      • VROAC antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on choroid plexus of mouse cerebrum (PMID: 18499744) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on mouse kidney (PMID:16627472, 18499744) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on rat kidney (PMID:16627472, 18499744) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on blood vessels of rat spleen (PMID:16627472) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    References

    ab231772 has not yet been referenced specifically in any publications.

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