Recombinant Anti-TRPV5 antibody [EPR8875] (ab137028)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8875] to TRPV5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TRPV5 antibody [EPR8875]
See all TRPV5 primary antibodies -
Description
Rabbit monoclonal [EPR8875] to TRPV5 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal kidney, Human small intestine, HepG2, 293T, Saos-2, Mouse heart, and Rat heart lysates. IHC-P: human breast and kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Dissociation constant (KD)
KD = 5.70 x 10 -13 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8875 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 83 kDa.
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IHC-P |
1/100 - 1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 83 kDa. |
IHC-P
1/100 - 1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating. -
Tissue specificity
Expressed at high levels in kidney, small intestine and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum. -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV5 sub-subfamily.
Contains 5 ANK repeats. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Apical cell membrane. Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells (By similarity). The expression of the glycosylated form in the cell membrane is increased in the presence of WNK3. - Information by UniProt
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Database links
- Entrez Gene: 56302 Human
- Entrez Gene: 194352 Mouse
- Entrez Gene: 116469 Rat
- Omim: 606679 Human
- SwissProt: Q9NQA5 Human
- SwissProt: P69744 Mouse
- SwissProt: Q9JIP0 Rat
- Unigene: 283369 Human
see all -
Alternative names
- Calcium transport protein 2 antibody
- Calcium transporter 2 antibody
- CAT 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling TRPV5 with purified ab137028 at 1:300 dilution (0.95 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-TRPV5 antibody [EPR8875] (ab137028) at 1/10000 dilution (Purified)
Lane 1 : Human fetal kidney lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling TRPV5 with unpurified ab137028 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-TRPV5 antibody [EPR8875] (ab137028) at 1/10000 dilution (unpurified)
Lane 1 : Fetal kidney lysate
Lane 2 : Human small intestine lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Saos-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 83 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TRPV5 with unpurified ab137028 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab137028 has been referenced in 5 publications.
- Cui Z et al. miR-103a-3p Silencing Ameliorates Calcium Oxalate Deposition in Rat Kidney by Activating the UMOD/TRPV5 Axis. Dis Markers 2022:2602717 (2022). PubMed: 35251369
- Tayyeb A et al. Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury. Cells 11:N/A (2022). PubMed: 35456008
- Yoon S et al. Usp9X Controls Ankyrin-Repeat Domain Protein Homeostasis during Dendritic Spine Development. Neuron 105:506-521.e7 (2020). PubMed: 31813652
- Ide N et al. In vivo evidence for a limited role of proximal tubular Klotho in renal phosphate handling. Kidney Int 90:348-362 (2016). PubMed: 27292223
- Yang B et al. Induction of TRPV5 expression by small activating RNA targeting gene promoter as a novel approach to regulate cellular calcium transportation. Life Sci 114:70-6 (2014). PubMed: 25139832