Overview

  • Product name

    Anti-TRPV5 antibody [EPR8875]
    See all TRPV5 primary antibodies
  • Description

    Rabbit monoclonal [EPR8875] to TRPV5
  • Host species

    Rabbit
  • Specificity

    The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of Human TRPV5.

  • Positive control

    • WB: Human fetal kidney, Human small intestine, HepG2, 293T, Saos-2, Mouse heart, and Rat heart lysates. IHC-P: human breast and kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Dissociation constant (KD)

    KD = 5.70 x 10 -13 M
    Learn more about KD
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8875
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 83 kDa.
IHC-P 1/100 - 1/300.

The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.
    • Tissue specificity

      Expressed at high levels in kidney, small intestine and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum.
    • Sequence similarities

      Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV5 sub-subfamily.
      Contains 5 ANK repeats.
    • Post-translational
      modifications

      Glycosylated.
    • Cellular localization

      Apical cell membrane. Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells (By similarity). The expression of the glycosylated form in the cell membrane is increased in the presence of WNK3.
    • Information by UniProt
    • Database links

    • Alternative names

      • Calcium transport protein 2 antibody
      • Calcium transporter 2 antibody
      • CAT 2 antibody
      • CAT2 antibody
      • ECAC 1 antibody
      • ECaC antibody
      • ECaC1 antibody
      • Epithelial calcium channel 1 antibody
      • Osm 9 like TRP channel 3 antibody
      • Osm-9-like TRP channel 3 antibody
      • OTRPC 3 antibody
      • OTRPC3 antibody
      • Transient receptor potential cation channel subfamily V member 5 antibody
      • TRPV 5 antibody
      • TrpV5 antibody
      • TRPV5_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling TRPV5 with purified ab137028 at 1:300 dilution (0.95 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • All lanes : Anti-TRPV5 antibody [EPR8875] (ab137028) at 1/10000 dilution (Purified)

      Lane 1 : Human fetal kidney lysates
      Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
      Lane 3 : Mouse heart lysates
      Lane 4 : Rat heart lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 83 kDa
      Observed band size: 90 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling TRPV5 with unpurified ab137028 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-TRPV5 antibody [EPR8875] (ab137028) at 1/10000 dilution (unpurified)

      Lane 1 : Fetal kidney lysate
      Lane 2 : Human small intestine lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Saos-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 83 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TRPV5 with unpurified ab137028 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Yang B  et al. Induction of TRPV5 expression by small activating RNA targeting gene promoter as a novel approach to regulate cellular calcium transportation. Life Sci 114:70-6 (2014). Read more (PubMed: 25139832) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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