Overview

  • Product name

    Anti-TRRAP antibody [EPR14952-81]
    See all TRRAP primary antibodies
  • Description

    Rabbit monoclonal [EPR14952-81] to TRRAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TRRAP aa 2700-2850. The exact sequence is proprietary.
    Database link: Q9Y4A5

  • Positive control

    • HeLa, Jukat, Molt-4 and K562 cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/1000 - 1/2000. Detects a band of approximately 438 kDa (predicted molecular weight: 438 kDa).

Target

  • Relevance

    TRRAP (TRansformation/tRanscription domain Associated Protein) is an adapter protein that is found in a number of multiprotein chromatin complexes with histone acetyltransferase (HAT) activity. These complexes include PCAF, TFTC-HAT, TIP60 HAT, STAGA, and BAF53 complexes. TRRAP is thought to be responsible for the concerted and context-dependent recruitment of these complexes to chromatin during transcription, replication and DNA repair. TRRAP plays a key role in transcriptional activation by c-Myc, p53/TP53, E2F1 and E2F4.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • 350/400 kDa PCAF associated factor antibody
    • PAF 350/400 antibody
    • PAF 400 antibody
    • PAF350/400 antibody
    • PAF400 antibody
    • STAF 40 antibody
    • STAF40 antibody
    • TR AP antibody
    • Tra 1 antibody
    • Tra1 antibody
    • Tra1 homolog antibody
    • Transactivation / transformation domain associated protein antibody
    • Transactivation/transformation domain associated protein antibody
    • Transformation/transcription domain associated protein antibody
    • TRAP antibody
    see all

Images

  • All lanes : Anti-TRRAP antibody [EPR14952-81] (ab187166) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Molt-4 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 438 kDa
    Observed band size: 438 kDa

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TRRAP with ab187166 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

References

ab187166 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab187166.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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