Overview

  • Product name

    Anti-TRUB2 antibody [EPR17326]
    See all TRUB2 primary antibodies
  • Description

    Rabbit monoclonal [EPR17326] to TRUB2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TRUB2 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: O95900

  • Positive control

    • A549, K562 and HepG2 whole cell lysates; Human fetal skin lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

Target

  • Function

    May be responsible for synthesis of pseudouridine from uracil in transfer RNAs.
  • Sequence similarities

    Belongs to the pseudouridine synthase TruB family.
  • Information by UniProt
  • Database links

  • Alternative names

    • CLONE24922 antibody
    • OTTHUMP00000022257 antibody
    • Probable tRNA pseudouridine synthase 2 antibody
    • RP11 339B21.1 antibody
    • TRUB 2 antibody
    • TruB pseudouridine (psi) synthase homolog 2 antibody
    • TruB pseudouridine (psi) synthase homolog2 (E. coli) antibody
    • trub2 antibody
    • TRUB2_HUMAN antibody
    see all

Images

  • All lanes : Anti-TRUB2 antibody [EPR17326] (ab199731) at 1/5000 dilution

    Lane 1 : A549 (Human lung carcinoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-TRUB2 antibody [EPR17326] (ab199731) at 1/1000 dilution + Human fetal skin lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab199731 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab199731.
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