Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651)
Key features and details
- Rabbit polyclonal to Trypanosoma brucei phosphoglycerate kinase
- Suitable for: ICC/IF, WB
- Reacts with: Trypanosoma brucei
- Isotype: IgG
Overview
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Product name
Anti-Trypanosoma brucei phosphoglycerate kinase antibody -
Description
Rabbit polyclonal to Trypanosoma brucei phosphoglycerate kinase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Trypanosoma brucei -
Immunogen
Synthetic peptide derived from the C terminal domain of Trypanosoma brucei phosphoglycerate kinase.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (1) |
1/200 - 1/2000. Predicted molecular weight: 55 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/200 - 1/2000. Predicted molecular weight: 55 kDa. |
Target
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Relevance
Belongs to the phosphoglycerate kinase family. -
Database links
- SwissProt: P08891 Trypanosoma brucei
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Alternative names
- PGK A allele 2 antibody
- Phosphoglycerate kinase A antibody
- Trypanosoma brucei PGK antibody
Images
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All lanes : Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651) at 1/200 dilution (in TBS + 5% milk for 12 hours at 4°C)
Lane 1 : Whole cell lysate of Trypanosoma brucei - procyclic form
Lane 2 : Whole cell lysate of Trypanosoma brucei - bloodstream
Lysates/proteins at 20000000 cells per lane.
Secondary
All lanes : A biotin-conjugated Goat anti-rabbit IgG polyclonal at 1/200 dilution
Predicted band size: 55 kDa
Observed band size: 65,75 kDa why is the actual band size different from the predicted?
Blocking Step:5% Milk for 1 hour at 25°C
Detection Method: DAB
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab89651 has not yet been referenced specifically in any publications.