Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651)

Overview

  • Product name

    Anti-Trypanosoma brucei phosphoglycerate kinase antibody
  • Description

    Rabbit polyclonal to Trypanosoma brucei phosphoglycerate kinase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, ICC, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide derived from the C terminal domain of Trypanosoma brucei phosphoglycerate kinase.

Properties

Applications

Our Abpromise guarantee covers the use of ab89651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent concentration.
WB 1/200 - 1/2000. Predicted molecular weight: 55 kDa.

Target

  • Relevance

    Belongs to the phosphoglycerate kinase family.
  • Alternative names

    • PGK A allele 2 antibody
    • Phosphoglycerate kinase A antibody
    • Trypanosoma brucei PGK antibody

Images

  • All lanes : Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651) at 1/200 dilution (in TBS + 5% milk for 12 hours at 4°C)

    Lane 1 : Whole cell lysate of Trypanosoma brucei - procyclic form
    Lane 2 : Whole cell lysate of Trypanosoma brucei - bloodstream

    Lysates/proteins at 20000000 cells per lane.

    Secondary
    All lanes : A biotin-conjugated Goat anti-rabbit IgG polyclonal at 1/200 dilution

    Predicted band size: 55 kDa
    Observed band size: 65,75 kDa
    why is the actual band size different from the predicted?



    Blocking Step:5% Milk for 1 hour at 25°C
    Detection Method: DAB

    See Abreview

References

ab89651 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Trypanosoma brucei Cell lysate - whole cell (Trypanosoma brucei procyclic form and bloodstream)
Loading amount
2e+007 cells
Specification
Trypanosoma brucei procyclic form and bloodstream
Gel Running Conditions
Reduced Denaturing (10% separating 4% stacking gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Susan Rouse

Verified customer

Submitted Aug 26 2010

Application
Immunocytochemistry
Sample
Trypanosoma brucei Cultured Cells (Blood stream and procyclic forms)
Specification
Blood stream and procyclic forms
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 37°C

Dr. Susan Rouse

Verified customer

Submitted Aug 25 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up