Key features and details
- Rabbit polyclonal to Trypanosoma brucei phosphoglycerate kinase
- Suitable for: ICC/IF, ICC, WB
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Trypanosoma brucei phosphoglycerate kinase antibody
DescriptionRabbit polyclonal to Trypanosoma brucei phosphoglycerate kinase
Tested applicationsSuitable for: ICC/IF, ICC, WBmore details
Species reactivityReacts with: Species independent
Synthetic peptide derived from the C terminal domain of Trypanosoma brucei phosphoglycerate kinase.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab89651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent concentration.|
|WB||1/200 - 1/2000. Predicted molecular weight: 55 kDa.|
RelevanceBelongs to the phosphoglycerate kinase family.
- PGK A allele 2 antibody
- Phosphoglycerate kinase A antibody
- Trypanosoma brucei PGK antibody
All lanes : Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651) at 1/200 dilution (in TBS + 5% milk for 12 hours at 4°C)
Lane 1 : Whole cell lysate of Trypanosoma brucei - procyclic form
Lane 2 : Whole cell lysate of Trypanosoma brucei - bloodstream
Lysates/proteins at 20000000 cells per lane.
All lanes : A biotin-conjugated Goat anti-rabbit IgG polyclonal at 1/200 dilution
Predicted band size: 55 kDa
Observed band size: 65,75 kDa why is the actual band size different from the predicted?
Blocking Step:5% Milk for 1 hour at 25°C
Detection Method: DAB
ab89651 has not yet been referenced specifically in any publications.