Recombinant Anti-Trypsin antibody [EPR19497] - BSA and Azide free (ab251325)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19497] to Trypsin - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Trypsin antibody [EPR19497] - BSA and Azide free
See all Trypsin primary antibodies -
Description
Rabbit monoclonal [EPR19497] to Trypsin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251325 is the carrier-free version of ab200996.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR19497 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates. -
Involvement in disease
Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsOccurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 5644 Human
- Entrez Gene: 5645 Human
- Entrez Gene: 5646 Human
- Omim: 276000 Human
- Omim: 601564 Human
- Omim: 613578 Human
- SwissProt: P07477 Human
- SwissProt: P07478 Human
see all -
Alternative names
- Alpha-trypsin chain 2 antibody
- Anionic trypsinogen antibody
- Beta trypsin antibody
see all
Images
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All lanes : Anti-Trypsin antibody [EPR19497] (ab200996) at 1/1000 dilution
Lane 1 : Human PRSS1 fragment recombinant protein
Lane 2 : Human TRY3 full length recombinant protein
Lane 3 : Human TRY6 full length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 27 kDaThis data was developed using ab200996, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 1 second; Lanes 2-3: 3 minutes.
Human PRSS1 fragment recombinant protein contains aa24-122 with a His-Tag®. Human TRY3 full length recombinant protein contains aa1-304 with a His-Tag®. Human TRY6 full length recombinant protein contains aa1-247 with a His-Tag®. These three recombinant proteins were made in-house.
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Anti-Trypsin antibody [EPR19497] (ab200996) at 1/1000 dilution + Human TRY2 full length recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 27 kDa
Exposure time: 3 minutesThis data was developed using ab200996, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human TRY2 full length recombinant protein contains aa1-247 with a GST-Tag. It was made in-house.
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All lanes : Anti-Trypsin antibody [EPR19497] (ab200996) at 1/5000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Human fetal heart tissue lysate
Lane 4 : Human fetal kidney tissue lysate
Lane 5 : Human fetal spleen tissue lysate
Lane 6 : Human fetal lung tissue lysate
Lane 7 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
Lane 8 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 9 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 3 minutesThis data was developed using ab200996, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Trypsin 1, encoded by the PRSS1 gene, is synthesized in the pancreas. The expression profile observed is consistent with what has been described in the literatures (PMID: 3011602, 7945238, 24722290).
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This data was developed using ab200996, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on acinar cells Human pancreas is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab200996, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251325 has not yet been referenced specifically in any publications.