Recombinant
RabMAb

Recombinant Anti-Trypsin antibody [EPR19498-15] (ab211491)

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Overview

  • Product name

    Anti-Trypsin antibody [EPR19498-15]
    See all Trypsin primary antibodies

  • Description

    Rabbit monoclonal [EPR19498-15] to Trypsin

  • Host species

    Rabbit

  • Specificity

    This product reacts with human Trypsin 1, Trypsin 2, Trypsin 3 and putative trypsin 6. It might cross react with other family members and isoforms due to high sequence homology.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Trypsin aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P07477

  • Positive control

    • WB: Human PRSS1 fragment recombinant protein; Human PRSS2 full length recombinant protein; Human PRSS3 full length recombinant protein; Human PRSS3P2 full length recombinant protein; Human pancreas lysate. IHC-P: Human pancreas tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab211491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.

  • Involvement in disease

    Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.

  • Post-translational
    modifications

    Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Alpha-trypsin chain 2 antibody
    • Anionic trypsinogen antibody
    • Beta trypsin antibody
    • Beta-trypsin antibody
    • Brain trypsinogen antibody
    • Cationic trypsinogen antibody
    • Digestive zymogen antibody
    • Mesotrypsin antibody
    • Mesotrypsinogen antibody
    • MGC111183 antibody
    • MGC120174 antibody
    • MGC120175 antibody
    • MGC149362 antibody
    • MTG antibody
    • Nonfunctional trypsin 1 antibody
    • Pancreatic trypsinogen III antibody
    • Protease serine 1 (trypsin 1) antibody
    • Protease Serine 1 antibody
    • Protease serine 2 (trypsin 2) antibody
    • Protease serine 2 antibody
    • Protease serine 2 preproprotein antibody
    • Protease serine 3 (mesotrypsin) antibody
    • Protease serine 3 antibody
    • Protease serine 4 (trypsin 4 brain) antibody
    • Prss1 antibody
    • PRSS2 antibody
    • PRSS3 antibody
    • PRSS4 antibody
    • Serine protease 1 antibody
    • Serine protease 2 antibody
    • Serine protease 3 antibody
    • Serine protease 4 antibody
    • T9 antibody
    • TCR V beta 4.1 antibody
    • TRP1 antibody
    • TRY1 antibody
    • TRY1_HUMAN antibody
    • TRY2 antibody
    • TRY3 antibody
    • TRY4 antibody
    • TRY8 antibody
    • TRYP1 antibody
    • TRYP2 antibody
    • Trypsin 1 antibody
    • Trypsin 2 antibody
    • Trypsin 3 antibody
    • Trypsin I antibody
    • Trypsin II antibody
    • Trypsin III antibody
    • Trypsin IV antibody
    • Trypsinogen 1 antibody
    • Trypsinogen 2 antibody
    • Trypsinogen 4 antibody
    • Trypsinogen 5 antibody
    • Trypsinogen A antibody
    • Trypsinogen IV antibody
    • Trypsinogen, anionic antibody
    • Trypsinogen, cationic antibody
    see all

Images

  • Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/1000 dilution

    Lane 1 : Human PRSS1 fragment recombinant protein
    Lane 2 : Human PRSS2 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa
    Observed band size: 40,53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human PRSS1 fragment recombinant protein contains aa123-247 with a GST-Tag; Human PRSS2 full length recombinant protein contains aa1-247 with a GST-Tag. These two recombinant proteins were made in-house.

  • Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/1000 dilution

    Lane 1 : Human PRSS3 full length recombinant protein
    Lane 2 : Human PRSS3P2 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa
    Observed band size: 28,38 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 5 seconds; Lane 2: 1 minute.

    Human PRSS3 full length recombinant protein contains aa1-304 with a His-Tag®. Human PRSS3P2 full length recombinant protein contains aa1-247 with a His-Tag®. These two recombinant proteins were made in-house.

  • Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/5000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal lung lysate
    Lane 5 : Human fetal spleen lysate
    Lane 6 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 2 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Trypsinogens I (PRSS1), Trypsinogens II (PRSS2), and mesotrypsinogen (PRSS3) are the major trypsinogen genes in the human pancreas. Trypsinogens I and II are the principal forms of trypsin that are secreted in pancreatic juice, while mesotrypsinogen is a minor trypsinogen in human pancreatic juice [PMID: 14726524, 3011602, 6698368, and 9099703]. PRSS3P2 is putative trypsin-6. This product recognizes all four trypsins. Pancreas is the positive tissue, while other non-related tissues are chosen as negative controls.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Trypsin with ab211491 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas acinar cells is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Trypsin with ab211491 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: negative staining on Human colon.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

Publishing research using ab211491? Please let us know so that we can cite the reference in this datasheet.

ab211491 has not yet been referenced specifically in any publications.

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