Recombinant Anti-Trypsin antibody [EPR19498-15] (ab211491)
![Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)](https://www.abcam.com/ps/products/211/ab211491/Images/ab211491-260170-anti-trypsin-antibody-epr19498-15-western-blot.jpg "Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19498-15] to Trypsin
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Trypsin antibody [EPR19498-15]
See all Trypsin primary antibodies -
Description
Rabbit monoclonal [EPR19498-15] to Trypsin -
Host species
Rabbit -
Specificity
This product reacts with human Trypsin 1, Trypsin 2, Trypsin 3 and putative trypsin 6. It might cross react with other family members and isoforms due to high sequence homology. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human PRSS1 fragment recombinant protein; Human PRSS2 full length recombinant protein; Human PRSS3 full length recombinant protein; Human PRSS3P2 full length recombinant protein; Human pancreas lysate. IHC-P: Human pancreas tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19498-15 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates. -
Involvement in disease
Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsOccurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 5644 Human
- Entrez Gene: 5645 Human
- Entrez Gene: 5646 Human
- Omim: 276000 Human
- Omim: 601564 Human
- Omim: 613578 Human
- SwissProt: P07477 Human
- SwissProt: P07478 Human
see all -
Alternative names
- Alpha-trypsin chain 2 antibody
- Anionic trypsinogen antibody
- Beta trypsin antibody
see all
Images
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Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/1000 dilution
Lane 1 : Human PRSS1 fragment recombinant protein
Lane 2 : Human PRSS2 full length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 27 kDa
Observed band size: 40,53 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Human PRSS1 fragment recombinant protein contains aa123-247 with a GST-Tag; Human PRSS2 full length recombinant protein contains aa1-247 with a GST-Tag. These two recombinant proteins were made in-house.
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![Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)](https://www.abcam.com/ps/products/211/ab211491/Images/ab211491-260169-anti-trypsin-antibody-epr19498-15-western-blot.jpg)
Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/1000 dilution
Lane 1 : Human PRSS3 full length recombinant protein
Lane 2 : Human PRSS3P2 full length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 27 kDa
Observed band size: 28,38 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 5 seconds; Lane 2: 1 minute.
Human PRSS3 full length recombinant protein contains aa1-304 with a His-Tag®. Human PRSS3P2 full length recombinant protein contains aa1-247 with a His-Tag®. These two recombinant proteins were made in-house.
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![Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)](https://www.abcam.com/ps/products/211/ab211491/Images/ab211491-260168-anti-trypsin-antibody-epr19498-15-western-blot.jpg)
Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/5000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human fetal lung lysate
Lane 5 : Human fetal spleen lysate
Lane 6 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 2 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Trypsinogens I (PRSS1), Trypsinogens II (PRSS2), and mesotrypsinogen (PRSS3) are the major trypsinogen genes in the human pancreas. Trypsinogens I and II are the principal forms of trypsin that are secreted in pancreatic juice, while mesotrypsinogen is a minor trypsinogen in human pancreatic juice [PMID: 14726524, 3011602, 6698368, and 9099703]. PRSS3P2 is putative trypsin-6. This product recognizes all four trypsins. Pancreas is the positive tissue, while other non-related tissues are chosen as negative controls.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)](https://www.abcam.com/ps/products/211/ab211491/Images/ab211491-260167-anti-trypsin-antibody-epr19498-15-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Trypsin with ab211491 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas acinar cells is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)](https://www.abcam.com/ps/products/211/ab211491/Images/ab211491-260166-anti-trypsin-antibody-epr19498-15-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Trypsin with ab211491 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: negative staining on Human colon.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-Trypsin antibody [EPR19498-15] (ab211491)](https://www.abcam.com/ps/products/211/ab211491/Images/ab211491-6-benefits-of-recombinant-antibodies.png)
Anti-Trypsin antibody [EPR19498-15] (ab211491)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab211491 has not yet been referenced specifically in any publications.