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    trypsin-antibody-epr19498-15-ab211491.pdf

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RecombinantRabMAb

Recombinant Anti-Trypsin antibody [EPR19498-15] (ab211491)

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  • SDS
  • Certificate of Compliance
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Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
  • Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
  • Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)
  • Anti-Trypsin antibody [EPR19498-15] (ab211491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19498-15] to Trypsin
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment

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Overview

  • Product name

    Anti-Trypsin antibody [EPR19498-15]
    See all Trypsin primary antibodies
  • Description

    Rabbit monoclonal [EPR19498-15] to Trypsin
  • Host species

    Rabbit
  • Specificity

    This product reacts with human Trypsin 1, Trypsin 2, Trypsin 3 and putative trypsin 6. It might cross react with other family members and isoforms due to high sequence homology.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human PRSS1 fragment recombinant protein; Human PRSS2 full length recombinant protein; Human PRSS3 full length recombinant protein; Human PRSS3P2 full length recombinant protein; Human pancreas lysate. IHC-P: Human pancreas tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19498-15
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-Trypsin antibody [EPR19498-15] - BSA and Azide free (ab251527)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Native cow Trypsin protein (ab229834)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab211491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
  • Involvement in disease

    Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.
  • Cellular localization

    Secreted > extracellular space.
  • Target information above from: UniProt accession P07477 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5644 Human
    • Entrez Gene: 5645 Human
    • Entrez Gene: 5646 Human
    • Omim: 276000 Human
    • Omim: 601564 Human
    • Omim: 613578 Human
    • SwissProt: P07477 Human
    • SwissProt: P07478 Human
    • SwissProt: P35030 Human
    • Unigene: 449281 Human
    see all
  • Alternative names

    • Alpha-trypsin chain 2 antibody
    • Anionic trypsinogen antibody
    • Beta trypsin antibody
    • Beta-trypsin antibody
    • Brain trypsinogen antibody
    • Cationic trypsinogen antibody
    • Digestive zymogen antibody
    • Mesotrypsin antibody
    • Mesotrypsinogen antibody
    • MGC111183 antibody
    • MGC120174 antibody
    • MGC120175 antibody
    • MGC149362 antibody
    • MTG antibody
    • Nonfunctional trypsin 1 antibody
    • Pancreatic trypsinogen III antibody
    • Protease serine 1 (trypsin 1) antibody
    • Protease Serine 1 antibody
    • Protease serine 2 (trypsin 2) antibody
    • Protease serine 2 antibody
    • Protease serine 2 preproprotein antibody
    • Protease serine 3 (mesotrypsin) antibody
    • Protease serine 3 antibody
    • Protease serine 4 (trypsin 4 brain) antibody
    • Prss1 antibody
    • PRSS2 antibody
    • PRSS3 antibody
    • PRSS4 antibody
    • Serine protease 1 antibody
    • Serine protease 2 antibody
    • Serine protease 3 antibody
    • Serine protease 4 antibody
    • T9 antibody
    • TCR V beta 4.1 antibody
    • TRP1 antibody
    • TRY1 antibody
    • TRY1_HUMAN antibody
    • TRY2 antibody
    • TRY3 antibody
    • TRY4 antibody
    • TRY8 antibody
    • TRYP1 antibody
    • TRYP2 antibody
    • Trypsin 1 antibody
    • Trypsin 2 antibody
    • Trypsin 3 antibody
    • Trypsin I antibody
    • Trypsin II antibody
    • Trypsin III antibody
    • Trypsin IV antibody
    • Trypsinogen 1 antibody
    • Trypsinogen 2 antibody
    • Trypsinogen 4 antibody
    • Trypsinogen 5 antibody
    • Trypsinogen A antibody
    • Trypsinogen IV antibody
    • Trypsinogen, anionic antibody
    • Trypsinogen, cationic antibody
    see all

Images

  • Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/1000 dilution

    Lane 1 : Human PRSS1 fragment recombinant protein
    Lane 2 : Human PRSS2 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa
    Observed band size: 40,53 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human PRSS1 fragment recombinant protein contains aa123-247 with a GST-Tag; Human PRSS2 full length recombinant protein contains aa1-247 with a GST-Tag. These two recombinant proteins were made in-house.

  • Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/1000 dilution

    Lane 1 : Human PRSS3 full length recombinant protein
    Lane 2 : Human PRSS3P2 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa
    Observed band size: 28,38 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 5 seconds; Lane 2: 1 minute.

    Human PRSS3 full length recombinant protein contains aa1-304 with a His-Tag®. Human PRSS3P2 full length recombinant protein contains aa1-247 with a His-Tag®. These two recombinant proteins were made in-house.

  • Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/5000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal lung lysate
    Lane 5 : Human fetal spleen lysate
    Lane 6 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 2 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Trypsinogens I (PRSS1), Trypsinogens II (PRSS2), and mesotrypsinogen (PRSS3) are the major trypsinogen genes in the human pancreas. Trypsinogens I and II are the principal forms of trypsin that are secreted in pancreatic juice, while mesotrypsinogen is a minor trypsinogen in human pancreatic juice [PMID: 14726524, 3011602, 6698368, and 9099703]. PRSS3P2 is putative trypsin-6. This product recognizes all four trypsins. Pancreas is the positive tissue, while other non-related tissues are chosen as negative controls.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Trypsin with ab211491 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas acinar cells is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Trypsin with ab211491 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: negative staining on Human colon.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Anti-Trypsin antibody [EPR19498-15] (ab211491)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab211491? Please let us know so that we can cite the reference in this datasheet.

ab211491 has not yet been referenced specifically in any publications.

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