Trypsin Antigen Retrieval Solution (ab970)
Overview
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Product name
Trypsin Antigen Retrieval Solution -
Tested applications
Suitable for: IHC-Pmore details -
General notes
Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970). Contains colored pH indicator for easy pH confirmation.
Protocol Notes: 1) Deparaffinize tissues and hydrate to water. If necessary perform a hydrogen peroxide block, wash in water, and rinse in PBS or TBS. The tissue section is ready for trypsin digestion. 2) Combine 1 part trypsin concentrate with 1 part buffer (working solution stable for 5 working days at 2-8ºC). Incubate section for 5-10 minutes at 37°C or 15 minutes at room temperature. Then, rinse well in PBS or TBS buffer and proceed with immunostaining procedure. This product utilises a color-coded pH indicator (rose) for easy identification. The end-user can visually inspect the solution and see that the mixture or buffer is at the proper pH. If the mixed solution is purple, the pH is too high for optimum digestion. If the buffer or trypsin solution turns orange or yellow, the pH is too low for optimum digestion.
Trypsin is a commonly used digestive enzyme. In formalin-fixed, paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining.
Other products for IHC
Other enzymes for antigen retrieval include: Proteinase K Antigen Retrieval Solution ab64220 and Pepsin Solution ab64201.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, Carrier protein -
Concentration information loading... -
Reagent notes
Trypsin is a commonly used digestive enzyme. In formalin-fixed, paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution. Ratio for 25ml concentrated trypsin and 25 ml buffer is 1:1 - 1:3.
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| Notes |
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IHC-P
Use at an assay dependent dilution. Ratio for 25ml concentrated trypsin and 25 ml buffer is 1:1 - 1:3. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab970 has been referenced in 24 publications.
- Xu Y et al. Identification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo. PLoS One 16:e0251386 (2021). PubMed: 33956893
- Choi MH et al. A selective small-molecule inhibitor of c-Met suppresses keloid fibroblast growth in vitro and in a mouse model. Sci Rep 11:5468 (2021). PubMed: 33750878
- Choi MC et al. Intra-Articular Administration of Cramp into Mouse Knee Joint Exacerbates Experimental Osteoarthritis Progression. Int J Mol Sci 22:N/A (2021). PubMed: 33810460
- Gross-Amat O et al. Characterization of a Topically Testable Model of Burn Injury on Human Skin Explants. Int J Mol Sci 21:N/A (2020). PubMed: 32971882
- Wang Y et al. PDGFRa marks distinct perivascular populations with different osteogenic potential within adipose tissue. Stem Cells 38:276-290 (2020). PubMed: 31742801