Product nameTrypsin Enzymatic Antigen Retrieval Solution
See all Antigen retrieval solution reagents
Tested applicationsSuitable for: IHC-Pmore details
Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970). Contains colored pH indicator for easy pH confirmation.
Protocol Notes: 1) Deparaffinize tissues and hydrate to water. If necessary perform a hydrogen peroxide block, wash in water, and rinse in PBS or TBS. The tissue section is ready for trypsin digestion. 2) Combine 1 part trypsin concentrate with 1 part buffer (working solution stable for 5 working days at 2-8ºC). Incubate section for 5-10 minutes at 37°C or 15 minutes at room temperature. Then, rinse well in PBS or TBS buffer and proceed with immunostaining procedure. This product utilises a color-coded pH indicator (rose) for easy identification. The end-user can visually inspect the solution and see that the mixture or buffer is at the proper pH. If the mixed solution is purple, the pH is too high for optimum digestion. If the buffer or trypsin solution turns orange or yellow, the pH is too low for optimum digestion.
Trypsin is a commonly used digestive enzyme. In formalin-fixed, paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining.
Other products for IHC
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Storage instructionsStore at +4°C.
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: PBS, Carrier protein, pH 7.3
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Reagent notesTrypsin is a commonly used digestive enzyme. In formalin-fixed, paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining.
Our Abpromise guarantee covers the use of ab970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution. Ratio for 25ml concentrated trypsin and 25 ml buffer is 1:1 - 1:3.|
This product has been referenced in:
- Choi MC et al. Alleviation of Murine Osteoarthritis by Cartilage-Specific Deletion of I?B?. Arthritis Rheumatol 70:1440-1449 (2018). Read more (PubMed: 29604191) »
- Hong HN et al. Cancer-associated fibroblasts promote gastric tumorigenesis through EphA2 activation in a ligand-independent manner. J Cancer Res Clin Oncol 144:1649-1663 (2018). Read more (PubMed: 29948146) »