Overview

  • Product name
    Anti-Trypsin Inhibitor antibody
    See all Trypsin Inhibitor primary antibodies
  • Description
    Rabbit polyclonal to Trypsin Inhibitor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Dot blot, ELISA, Conjugationmore details
  • Species reactivity
    Reacts with: Soybean
  • Immunogen

    Full length protein [Soybean]

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
ELISA
Conjugation
  • Application notes
    Conjugation: Use at an assay dependent dilution.
    Dot: Use at an assay dependent dilution.
    ELISA: 1/3000 - 1/12000. This product has been assayed against 1.0µg of Trypsin Inhibitor [Soybean] in a standard sandwich ELISA using Peroxidase conjugated affinity purified anti-Rabbit IgG and ABTS as a substrate for 30 minutes at room temperature.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The soybean trypsin inhibitor was first crystallized by Kunitz in 1945 and is one of several such inhibitors found in soybeans. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly inhibited by soy inhibitor.
    • Database links
    • Alternative names
      • CRISP8 antibody
      • KTi antibody
      • KTi3 antibody
      • Kunitz trypsin inhibitor antibody
      • Kunitz type trypsin inhibitor A antibody
      • Trypsin inhibitor A antibody
      see all

    References

    This product has been referenced in:
    See all 2 Publications for this product

    Customer reviews and Q&As

    Question
    Answer

    I am very pleased to hear you would like to accept our offer and test ab34549 in IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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