Product nameAnti-Trypsin Inhibitor antibody
See all Trypsin Inhibitor primary antibodies
DescriptionRabbit polyclonal to Trypsin Inhibitor
Tested applicationsSuitable for: WB, Dot blot, ELISA, Conjugationmore details
Species reactivityReacts with: Soybean
Full length protein [Soybean]
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Our Abpromise guarantee covers the use of ab34549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
ELISA: 1/3000 - 1/12000. This product has been assayed against 1.0µg of Trypsin Inhibitor [Soybean] in a standard sandwich ELISA using Peroxidase conjugated affinity purified anti-Rabbit IgG and ABTS as a substrate for 30 minutes at room temperature.
WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe soybean trypsin inhibitor was first crystallized by Kunitz in 1945 and is one of several such inhibitors found in soybeans. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly inhibited by soy inhibitor.
- CRISP8 antibody
- KTi antibody
- KTi3 antibody
This product has been referenced in:
- Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A 109:21450-5 (2012). WB . Read more (PubMed: 23223527) »
- Hope-Onyekwere NS et al. Effects of Mucuna pruriens Protease Inhibitors on Echis carinatus Venom. Phytother Res 26:1913-9 (2012). Read more (PubMed: 22447581) »