Recombinant Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1311Y] to Tryptophan Hydroxylase/TPH
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y]
See all Tryptophan Hydroxylase/TPH primary antibodies -
Description
Rabbit monoclonal [EP1311Y] to Tryptophan Hydroxylase/TPH -
Host species
Rabbit -
Specificity
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human small intestine and pituitary tissue lysate. Mouse brain tissue lysate. Recombinant full length mouse Tph1 protein with C-terminal MYC/DDK tag. N-His tagged human Tryptophan Hydroxylase/TPH (aa 105 to 420) recombinant protein
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1311Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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IHC-P | (2) |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. For the unpurified format use at 1/50 dilution |
Notes |
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WB
1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. For the unpurified format use at 1/50 dilution |
Target
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Tissue specificity
Isoform 2 seems to be less widely expressed than isoform 1. -
Pathway
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain. - Information by UniProt
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Database links
- Entrez Gene: 7166 Human
- Entrez Gene: 21990 Mouse
- Omim: 191060 Human
- SwissProt: P17752 Human
- SwissProt: P17532 Mouse
- Unigene: 591999 Human
- Unigene: 248684 Mouse
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Alternative names
- Indoleacetic acid 5 hydroxylase antibody
- L tryptophan hydroxylase antibody
- MGC119994 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Tryptophan Hydroxylase/TPH with Purified ab52954 at 1:2000 dilution (1.191 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Lane 1 : Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution
Lane 2 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/1000 dilution
All lanes : Full-length Tph1 mouse recombinant protein with C-terminal MYC/DDK tag 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution
Lane 1 : Human small intestine tissue lysate
Lane 2 : Human pituitary tissue lysate
Lane 3 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 180 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
TPH1 is mainly expressed in non-neuronal peripheral tissues like pineal gland.
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Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution + N-His tagged human Tryptophan Hydroxylase/TPH (aa 105 to 420) recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab52954 has been referenced in 24 publications.
- Omoluabi T et al. Novelty-like activation of locus coeruleus protects against deleterious human pretangle tau effects while stress-inducing activation worsens its effects. Alzheimers Dement (N Y) 7:e12231 (2021). PubMed: 35005208
- Yi G et al. A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse model. RSC Adv 10:11264-11273 (2020). PubMed: 35495343
- Chen DQ et al. Poricoic acid A as a modulator of TPH-1 expression inhibits renal fibrosis via modulating protein stability of ß-catenin and ß-catenin-mediated transcription. Ther Adv Chronic Dis 11:2040622320962648 (2020). PubMed: 33062239
- Cheng Q et al. [6]-Gingerol Ameliorates Cisplatin-Induced Pica by Regulating the TPH/MAO-A/SERT/5-HT/5-HT3 Receptor System in Rats. Drug Des Devel Ther 14:4085-4099 (2020). PubMed: 33061309
- Chen DQ et al. Identification of serum metabolites associating with chronic kidney disease progression and anti-fibrotic effect of 5-methoxytryptophan. Nat Commun 10:1476 (2019). PubMed: 30931940