Key features and details
- Rabbit polyclonal to Tryptophan Hydroxylase/TPH
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Tryptophan Hydroxylase/TPH antibody
See all Tryptophan Hydroxylase/TPH primary antibodies
DescriptionRabbit polyclonal to Tryptophan Hydroxylase/TPH
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Dog, Xenopus laevis
Recombinant fragment within Human Tryptophan Hydroxylase/TPH (internal sequence). The exact sequence is proprietary.
Database link: P17752
- WB: HepG2 whole cell lysate. ICC/IF: SK-N-SH cells.
This product was previously labelled as Tryptophan Hydroxylase
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab228588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 51 kDa.|
|ICC/IF||1/100 - 1/1000.|
Tissue specificityIsoform 2 seems to be less widely expressed than isoform 1.
PathwayAromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain.
- Information by UniProt
- Indoleacetic acid 5 hydroxylase antibody
- L tryptophan hydroxylase antibody
- MGC119994 antibody
Anti-Tryptophan Hydroxylase/TPH antibody (ab228588) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
SK-N-SH (human neuroblastoma cell line) cells stained for Tryptophan Hydroxylase/TPH (green) using ab228588 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228588 has not yet been referenced specifically in any publications.