Product nameAnti-Tryptophan Hydroxylase/TPH antibody
See all Tryptophan Hydroxylase/TPH primary antibodies
DescriptionRabbit polyclonal to Tryptophan Hydroxylase/TPH
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Dog, Xenopus laevis
Recombinant fragment within Human Tryptophan Hydroxylase/TPH (internal sequence). The exact sequence is proprietary.
Database link: P17752
- WB: HepG2 whole cell lysate. ICC/IF: SK-N-SH cells.
This product was previously labelled as Tryptophan Hydroxylase
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab228588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 51 kDa.|
|ICC/IF||1/100 - 1/1000.|
Tissue specificityIsoform 2 seems to be less widely expressed than isoform 1.
PathwayAromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain.
- Information by UniProt
- Indoleacetic acid 5 hydroxylase antibody
- L tryptophan hydroxylase antibody
- MGC119994 antibody
Anti-Tryptophan Hydroxylase/TPH antibody (ab228588) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
SK-N-SH (human neuroblastoma cell line) cells stained for Tryptophan Hydroxylase/TPH (green) using ab228588 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
ab228588 has not yet been referenced specifically in any publications.