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    tryptophan-hydroxylasetph-antibody-ab32821.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-Tryptophan Hydroxylase/TPH antibody (ab32821)

  • Datasheet
Reviews (1)Q&A (3)

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Western blot - Anti-Tryptophan Hydroxylase/TPH antibody (ab32821)

    Key features and details

    • Sheep polyclonal to Tryptophan Hydroxylase/TPH
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Tryptophan Hydroxylase/TPH antibody
      See all Tryptophan Hydroxylase/TPH primary antibodies
    • Description

      Sheep polyclonal to Tryptophan Hydroxylase/TPH
    • Host species

      Sheep
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: a wide range of other species
    • Immunogen

      Recombinant full length protein corresponding to Rabbit Tryptophan Hydroxylase/TPH.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.50
      Constituents: 0.238% HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Neuroscience
      • Neurotransmitter
      • Amino Acids
      • Tryptophan
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Hormone biosynthesis
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Amino acid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Pathways and Processes
      • Endocrine metabolism
      • Hormone biosynthesis
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Isotype control

      • Sheep IgG - Isotype Control (ab37385)
    • Recombinant Protein

      • Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (ab206448)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab32821 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
    Notes
    WB
    1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

    Target

    • Tissue specificity

      Isoform 2 seems to be less widely expressed than isoform 1.
    • Pathway

      Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
    • Sequence similarities

      Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Contains 1 ACT domain.
    • Target information above from: UniProt accession P17752 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7166 Human
      • Omim: 191060 Human
      • SwissProt: P17752 Human
      • Unigene: 591999 Human
      • Alternative names

        • Indoleacetic acid 5 hydroxylase antibody
        • L tryptophan hydroxylase antibody
        • MGC119994 antibody
        • TPH 1 antibody
        • TPH antibody
        • TPH1 antibody
        • TPH1_HUMAN antibody
        • TPRH antibody
        • TRPH antibody
        • Tryptophan 5 hydroxylase 1 antibody
        • Tryptophan 5 monooxygenase 1 antibody
        • Tryptophan 5 monooxygenase antibody
        • Tryptophan 5-hydroxylase 1 antibody
        • Tryptophan 5-monooxygenase 1 antibody
        • Tryptophan hydroxylase 1 antibody
        see all

      Images

      • Western blot - Anti-Tryptophan Hydroxylase/TPH antibody (ab32821)
        Western blot - Anti-Tryptophan Hydroxylase/TPH antibody (ab32821)
        Anti-Tryptophan Hydroxylase/TPH antibody (ab32821) at 1/1000 dilution + Human dorsal Raphe nucleus extract at 50 µg

        Secondary
        Anti-Sheep HRP labelled.

        Predicted band size: 51 kDa
        Observed band size: 55 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab32821? Please let us know so that we can cite the reference in this datasheet.

      ab32821 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-Tryptophan Hydroxylase/TPH antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (intestine)
      Permeabilization
      Yes - 0.25% Triton X-100
      Specification
      intestine
      Fixative
      Paraformaldehyde
      Read More

      MISS. Xiaowen Cheng

      Verified customer

      Submitted Feb 13 2018

      Question

      Hi, I was wondering if you could tell me the concentrations of ab32821 and ab101853.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 25 2012

      Answer

      Thank you for your inquiry and I apologize for the delay while I was awaiting more information from the lab.

      For ab32821, the concentration is 0.17 mg/ml.

      For ab101853, this antibody is a whole antiserum and is therefore not purified so no concentration is determined. Generally for this type of preparation, protein concentration ranges from 1 to 10 mg/ml if you need to estimate.

      I hope this information helps. Please contact us with any other questions.

      Read More

      Abcam Scientific Support

      Answered on Jun 25 2012

      Question

      Dear
      I have only now noticed that the customer hasn't s submitted the review yet.
      The customer has had unexpected delays in the research and has only recently received results for the abreview.
      We try to inform our customers of the expiry date and to give a notice prior to the last date. We would very much appreciate it if you could prolong the expiry date and allow the customer to submit the review.
      Thank you for your kind help.
      Regards,

      Read More

      Abcam community

      Verified customer

      Asked on Apr 03 2012

      Answer

      Thank you for contacting us.

      This is not something that we do on a regular basis. In this case I can make an exception if the customer is ready to submit the Abreview before the end of next week.

      If this is the case, please let me know and I will talk to my Customer Service colleagues once they receive your new free order in order to extend the expiration date.

      Do not hesitate to contact us for further assistance.

      Read More

      Abcam Scientific Support

      Answered on Apr 03 2012

      Question

      Sehr geehrte Damen und Herren,

      ich interessiere mich für das Produkt ab32821 (sheep polyclonal anti-tryptophan hydroxylase).
      Da ich vorhabe IHC-Färbungen auf Maushirnschnitten durchzuführen, frage ich mich, wie gut dieser Antikörper murines Tph2-Protein erkennt. Könnten Sie mir die entsprechende Epitopsequenz nennen (oder zumindest sagen, zu wieviel Prozent das Eptitop mit muriner Tph2 übereinstimmt)?

      Vielen Dank!

      Beste Grüße,

      Read More

      Abcam community

      Verified customer

      Asked on Mar 30 2012

      Answer

      Vielen Dank für Ihre Anfrage und für Ihr Interesse an diesem Antikörper.
      Dieser Antikörper ist ein polyklonaler Antikörper welcher gegen das ganze Protein produziert wurder. Die Epitope können daher theoretisch überall auf dem Protein sein. Da jedoch das ganze Protein relativ homolog mit der Tryptophan Hydroxylase aus der Maus ist, ist die Chance gross, dass der Antikörper auch das Maus Protein erkennt. Hier ist ein Link zu dem Sequenz Alignment:
      http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120330-085614-0085-81124694-oy
      Da unseres Wissens nach wurde ab32821 jedoch bisher noch nicht experimentell auf Mausgewebeschnitten getestet worden ist, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten, falls Sie dies selbst testen möchten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab32821 abdecken, und gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden.

      Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

      1.) Bestätigen Sie mir bitte, dass Sie ab32821 in Maus testen möchten.

      2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

      3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

      4.) Testen Sie den Antikörper in Maus.

      5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
      Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
      https://www.abcam.com/abreviews

      6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderen primären Antikörpers zu dem gleichen Preis wie ab32821 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab32821 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

      Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

      Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
      https://www.abcam.com/collaborationdiscount

      Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

      Read More

      Abcam Scientific Support

      Answered on Mar 30 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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