Anti-Tryptophan Hydroxylase/TPH antibody (ab32821)
Key features and details
- Sheep polyclonal to Tryptophan Hydroxylase/TPH
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Tryptophan Hydroxylase/TPH antibody
See all Tryptophan Hydroxylase/TPH primary antibodies -
Description
Sheep polyclonal to Tryptophan Hydroxylase/TPH -
Host species
Sheep -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: a wide range of other species
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Immunogen
Recombinant full length protein corresponding to Rabbit Tryptophan Hydroxylase/TPH.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). |
Target
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Tissue specificity
Isoform 2 seems to be less widely expressed than isoform 1. -
Pathway
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain. - Information by UniProt
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Database links
- Entrez Gene: 7166 Human
- Omim: 191060 Human
- SwissProt: P17752 Human
- Unigene: 591999 Human
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Alternative names
- Indoleacetic acid 5 hydroxylase antibody
- L tryptophan hydroxylase antibody
- MGC119994 antibody
see all
Images
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Western blot - Anti-Tryptophan Hydroxylase/TPH antibody (ab32821)Anti-Tryptophan Hydroxylase/TPH antibody (ab32821) at 1/1000 dilution + Human dorsal Raphe nucleus extract at 50 µg
Secondary
Anti-Sheep HRP labelled.
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab32821 has not yet been referenced specifically in any publications.