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    tryptophan-hydroxylasetph-antibody-ab32821.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-Tryptophan Hydroxylase/TPH antibody (ab32821)

  • Datasheet
Reviews (1)Q&A (3)

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Western blot - Anti-Tryptophan Hydroxylase/TPH antibody (ab32821)

    Key features and details

    • Sheep polyclonal to Tryptophan Hydroxylase/TPH
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Tryptophan Hydroxylase/TPH antibody
      See all Tryptophan Hydroxylase/TPH primary antibodies
    • Description

      Sheep polyclonal to Tryptophan Hydroxylase/TPH
    • Host species

      Sheep
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: a wide range of other species
    • Immunogen

      Recombinant full length protein corresponding to Rabbit Tryptophan Hydroxylase/TPH.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.50
      Constituents: 0.238% HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Neuroscience
      • Neurotransmitter
      • Amino Acids
      • Tryptophan
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Hormone biosynthesis
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Amino acid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Pathways and Processes
      • Endocrine metabolism
      • Hormone biosynthesis
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Isotype control

      • Sheep IgG - Isotype Control (ab37385)
    • Recombinant Protein

      • Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (ab206448)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab32821 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
    Notes
    WB
    1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

    Target

    • Tissue specificity

      Isoform 2 seems to be less widely expressed than isoform 1.
    • Pathway

      Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
    • Sequence similarities

      Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Contains 1 ACT domain.
    • Target information above from: UniProt accession P17752 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7166 Human
      • Omim: 191060 Human
      • SwissProt: P17752 Human
      • Unigene: 591999 Human
      • Alternative names

        • Indoleacetic acid 5 hydroxylase antibody
        • L tryptophan hydroxylase antibody
        • MGC119994 antibody
        • TPH 1 antibody
        • TPH antibody
        • TPH1 antibody
        • TPH1_HUMAN antibody
        • TPRH antibody
        • TRPH antibody
        • Tryptophan 5 hydroxylase 1 antibody
        • Tryptophan 5 monooxygenase 1 antibody
        • Tryptophan 5 monooxygenase antibody
        • Tryptophan 5-hydroxylase 1 antibody
        • Tryptophan 5-monooxygenase 1 antibody
        • Tryptophan hydroxylase 1 antibody
        see all

      Images

      • Western blot - Anti-Tryptophan Hydroxylase/TPH antibody (ab32821)
        Western blot - Anti-Tryptophan Hydroxylase/TPH antibody (ab32821)
        Anti-Tryptophan Hydroxylase/TPH antibody (ab32821) at 1/1000 dilution + Human dorsal Raphe nucleus extract at 50 µg

        Secondary
        Anti-Sheep HRP labelled.

        Predicted band size: 51 kDa
        Observed band size: 55 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab32821? Please let us know so that we can cite the reference in this datasheet.

      ab32821 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Immunohistochemistry (Frozen sections) abreview for Anti-Tryptophan Hydroxylase/TPH antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (intestine)
      Permeabilization
      Yes - 0.25% Triton X-100
      Specification
      intestine
      Fixative
      Paraformaldehyde
      Read More

      Miss. Xiaowen Cheng

      Verified customer

      Submitted Feb 13 2018

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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