Product nameAnti-Tryptophanyl tRNA synthetase / WRS antibody
See all Tryptophanyl tRNA synthetase / WRS primary antibodies
DescriptionRabbit polyclonal to Tryptophanyl tRNA synthetase / WRS
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human Tryptophanyl tRNA synthetase/ WRS.
- human normal breast tissue. HEK293 cell line.
Previously labelled as Tryptophanyl tRNA synthetase.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab31536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
|ICC/IF||1/50 - 1/1000.|
|IP||Use at an assay dependent concentration.|
FunctionIsoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 WHEP-TRS domain.
modificationsProteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
- Information by UniProt
- Gamma 2 antibody
- GAMMA-2 antibody
- Gamma2 antibody
Anti-Tryptophanyl tRNA synthetase / WRS antibody (ab31536) at 1/5000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml
Goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
ab31536 staining Tryptophanyl tRNA synthetase / WRS in HeLa cells by Immunocytochemistry/ Immunofluorescence.
This product has been referenced in:
- Norris EL et al. Proteoform-Specific Insights into Cellular Proteome Regulation. Mol Cell Proteomics 15:3297-3320 (2016). WB ; Human . Read more (PubMed: 27451424) »
- Al-Gubory KH et al. Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy. Reproduction 147:599-614 (2014). WB, IHC-P ; Sheep . Read more (PubMed: 24446454) »