Anti-Tryptophanyl tRNA synthetase / WRS antibody - Conformation-Specific (ab173801)

Overview

  • Product name
    Anti-Tryptophanyl tRNA synthetase / WRS antibody - Conformation-Specific
    See all Tryptophanyl tRNA synthetase / WRS primary antibodies
  • Description
    Rabbit polyclonal to Tryptophanyl tRNA synthetase / WRS - Conformation-Specific
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human Tryptophanyl tRNA synthetase/ WRS aa 228-258 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P23381

  • Positive control
    • Jurkat cell line lysates.
  • General notes

    Previously labelled as Tryptophanyl tRNA synthetase. 

Properties

Applications

Our Abpromise guarantee covers the use of ab173801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 53 kDa.

Target

  • Function
    Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
  • Sequence similarities
    Belongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 WHEP-TRS domain.
  • Post-translational
    modifications
    Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gamma 2 antibody
    • GAMMA-2 antibody
    • Gamma2 antibody
    • hWRS antibody
    • IFI 53 antibody
    • IFI53 antibody
    • IFP 53 antibody
    • IFP53 antibody
    • Interferon induced protein 53 antibody
    • Interferon-induced protein 53 antibody
    • SYWC_HUMAN antibody
    • T2-TrpRS antibody
    • TrpRS antibody
    • Tryptophan tRNA ligase, cytoplasmic antibody
    • Tryptophan tRNA ligase 1 cytoplasmic antibody
    • Tryptophan tRNA ligase antibody
    • Tryptophan--tRNA ligase antibody
    • Tryptophanyl tRNA synthetase antibody
    • Tryptophanyl tRNA synthetase cytoplasmic antibody
    • WARS antibody
    • WARS protein antibody
    • WRS antibody
    see all

Images

  • Anti-MED29 antibody - C-terminal (ab173501) at 1/100 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size: 53 kDa

References

ab173801 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Neurospora crassa Cell lysate - whole cell (Whole cell)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Specification
Whole cell
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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