Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724)
Key features and details
- Rabbit polyclonal to Tryptophanyl tRNA synthetase/WRS
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Tryptophanyl tRNA synthetase/WRS antibody
See all Tryptophanyl tRNA synthetase/WRS primary antibodies -
Description
Rabbit polyclonal to Tryptophanyl tRNA synthetase/WRS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Chimpanzee
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Immunogen
Recombinant fragment within Human Tryptophanyl tRNA synthetase/WRS (internal sequence). The exact sequence is proprietary.
Database link: P23381 -
Positive control
- IHC-P: U87 xenograft and C2C12 xenograft tissues. WB: A431 whole cell lysate.
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General notes
Previously labelled as Tryptophanyl tRNA synthetase.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab228724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 53 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. -
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 WHEP-TRS domain. -
Post-translational
modificationsProteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 453161 Chimpanzee
- Entrez Gene: 281576 Cow
- Entrez Gene: 7453 Human
- Entrez Gene: 22375 Mouse
- Entrez Gene: 100009123 Rabbit
- Entrez Gene: 314442 Rat
- Omim: 191050 Human
- SwissProt: P17248 Cow
see all -
Alternative names
- Gamma 2 antibody
- GAMMA-2 antibody
- Gamma2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724)Paraffin-embedded U87 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724)Paraffin-embedded C2C12 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724)Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724) at 1/10000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 50 µg
Predicted band size: 53 kDa10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab228724 has not yet been referenced specifically in any publications.
