Overview

  • Product name

    Anti-Tryptophanyl tRNA synthetase/WRS antibody
    See all Tryptophanyl tRNA synthetase/WRS primary antibodies
  • Description

    Rabbit polyclonal to Tryptophanyl tRNA synthetase/WRS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Chimpanzee
  • Immunogen

    Recombinant fragment within Human Tryptophanyl tRNA synthetase/WRS (internal sequence). The exact sequence is proprietary.
    Database link: P23381

  • Positive control

    • IHC-P: U87 xenograft and C2C12 xenograft tissues. WB: A431 whole cell lysate.
  • General notes

    Previously labelled as Tryptophanyl tRNA synthetase. 

Properties

Applications

Our Abpromise guarantee covers the use of ab228724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
  • Sequence similarities

    Belongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 WHEP-TRS domain.
  • Post-translational
    modifications

    Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Gamma 2 antibody
    • GAMMA-2 antibody
    • Gamma2 antibody
    • hWRS antibody
    • IFI 53 antibody
    • IFI53 antibody
    • IFP 53 antibody
    • IFP53 antibody
    • Interferon induced protein 53 antibody
    • Interferon-induced protein 53 antibody
    • SYWC_HUMAN antibody
    • T2-TrpRS antibody
    • TrpRS antibody
    • Tryptophan tRNA ligase, cytoplasmic antibody
    • Tryptophan tRNA ligase 1 cytoplasmic antibody
    • Tryptophan tRNA ligase antibody
    • Tryptophan--tRNA ligase antibody
    • Tryptophanyl tRNA synthetase antibody
    • Tryptophanyl tRNA synthetase cytoplasmic antibody
    • WARS antibody
    • WARS protein antibody
    • WRS antibody
    see all

Images

  • Paraffin-embedded U87 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded C2C12 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.

  • Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724) at 1/10000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 50 µg

    Predicted band size: 53 kDa



    10% SDS-PAGE gel.

References

ab228724 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9
Permeabilization
No
Specification
Kidney
Blocking step
Hydrogen Peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde

Fabian Lean

Verified customer

Submitted Mar 08 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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