Key features and details
- Rabbit polyclonal to Tryptophanyl tRNA synthetase/WRS
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Tryptophanyl tRNA synthetase/WRS antibody
See all Tryptophanyl tRNA synthetase/WRS primary antibodies
DescriptionRabbit polyclonal to Tryptophanyl tRNA synthetase/WRS
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Chimpanzee
Recombinant fragment within Human Tryptophanyl tRNA synthetase/WRS (internal sequence). The exact sequence is proprietary.
Database link: P23381
- IHC-P: U87 xenograft and C2C12 xenograft tissues. WB: A431 whole cell lysate.
Previously labelled as Tryptophanyl tRNA synthetase.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 53 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionIsoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 WHEP-TRS domain.
modificationsProteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
- Information by UniProt
- Gamma 2 antibody
- GAMMA-2 antibody
- Gamma2 antibody
Paraffin-embedded U87 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded C2C12 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.
Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724) at 1/10000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 50 µg
Predicted band size: 53 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228724 has not yet been referenced specifically in any publications.