Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Tryptophanyl tRNA synthetase/WRS antibody
See all Tryptophanyl tRNA synthetase/WRS primary antibodies -
Description
Rabbit polyclonal to Tryptophanyl tRNA synthetase/WRS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Chimpanzee
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Immunogen
Recombinant fragment within Human Tryptophanyl tRNA synthetase/WRS (internal sequence). The exact sequence is proprietary.
Database link: P23381 -
Positive control
- IHC-P: U87 xenograft and C2C12 xenograft tissues. WB: A431 whole cell lysate.
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General notes
Previously labelled as Tryptophanyl tRNA synthetase.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab228724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 53 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. -
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 WHEP-TRS domain. -
Post-translational
modificationsProteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 453161 Chimpanzee
- Entrez Gene: 281576 Cow
- Entrez Gene: 7453 Human
- Entrez Gene: 22375 Mouse
- Entrez Gene: 100009123 Rabbit
- Entrez Gene: 314442 Rat
- Omim: 191050 Human
- SwissProt: P17248 Cow
see all -
Alternative names
- Gamma 2 antibody
- GAMMA-2 antibody
- Gamma2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724)Paraffin-embedded U87 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724)Paraffin-embedded C2C12 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724)Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724) at 1/10000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 50 µg
Predicted band size: 53 kDa10% SDS-PAGE gel.
References
ab228724 has not yet been referenced specifically in any publications.
