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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs
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RecombinantRabMAb

Recombinant Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)

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Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
  • Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3423] to Tryptophanyl tRNA synthetase/WRS
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423]
    See all Tryptophanyl tRNA synthetase/WRS primary antibodies
  • Description

    Rabbit monoclonal [EPR3423] to Tryptophanyl tRNA synthetase/WRS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, JAR, 293T and L6 cell lysates; Human breast tissue
  • General notes

     

     

    Previously labelled as Tryptophanyl tRNA synthetase. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3423
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNA synthetase

Associated products

  • Alternative Versions

    • Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] - BSA and Azide free (ab247793)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (ab185823)
    • Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (ab99263)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
    • Sequence similarities

      Belongs to the class-I aminoacyl-tRNA synthetase family.
      Contains 1 WHEP-TRS domain.
    • Post-translational
      modifications

      Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P23381 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7453 Human
      • Entrez Gene: 22375 Mouse
      • Entrez Gene: 314442 Rat
      • Omim: 191050 Human
      • SwissProt: P23381 Human
      • SwissProt: P32921 Mouse
      • SwissProt: Q6P7B0 Rat
      • Unigene: 497599 Human
      • Unigene: 38433 Mouse
      • Unigene: 14939 Rat
      see all
    • Alternative names

      • Gamma 2 antibody
      • GAMMA-2 antibody
      • Gamma2 antibody
      • hWRS antibody
      • IFI 53 antibody
      • IFI53 antibody
      • IFP 53 antibody
      • IFP53 antibody
      • Interferon induced protein 53 antibody
      • Interferon-induced protein 53 antibody
      • SYWC_HUMAN antibody
      • T2-TrpRS antibody
      • TrpRS antibody
      • Tryptophan tRNA ligase, cytoplasmic antibody
      • Tryptophan tRNA ligase 1 cytoplasmic antibody
      • Tryptophan tRNA ligase antibody
      • Tryptophan--tRNA ligase antibody
      • Tryptophanyl tRNA synthetase antibody
      • Tryptophanyl tRNA synthetase cytoplasmic antibody
      • WARS antibody
      • WARS protein antibody
      • WRS antibody
      see all

    Images

    • Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
      Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
      All lanes : Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : L6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 53 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)

      ab109213, at 1/500 dilution, staining Tryptophanyl tRNA synthetase/WRS in paraffin-embedded Human breast tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
      Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab109213? Please let us know so that we can cite the reference in this datasheet.

    ab109213 has not yet been referenced specifically in any publications.

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