Recombinant Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423]
See all Tryptophanyl tRNA synthetase/WRS primary antibodies -
Description
Rabbit monoclonal [EPR3423] to Tryptophanyl tRNA synthetase/WRS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Tryptophanyl tRNA synthetase/WRS aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa, JAR, 293T and L6 cell lysates; Human breast tissue
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General notes
Previously labelled as Tryptophanyl tRNA synthetase.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3423 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 53 kDa. | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
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| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. -
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 WHEP-TRS domain. -
Post-translational
modificationsProteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7453 Human
- Entrez Gene: 22375 Mouse
- Entrez Gene: 314442 Rat
- Omim: 191050 Human
- SwissProt: P23381 Human
- SwissProt: P32921 Mouse
- SwissProt: Q6P7B0 Rat
- Unigene: 497599 Human
see all -
Alternative names
- Gamma 2 antibody
- GAMMA-2 antibody
- Gamma2 antibody
see all
Images
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![Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)](https://www.abcam.com/ps/products/109/ab109213/Images/ab109213-121842-anti-tryptophanyl-trna-synthetase-wrs-antibody-epr3423-western-blot.jpg)
Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)All lanes : Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : 293T cell lysate
Lane 4 : L6 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)](https://www.abcam.com/ps/products/109/ab109213/Images/ab109213-121841-anti-tryptophanyl-trna-synthetase-wrs-antibody-epr3423-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)ab109213, at 1/500 dilution, staining Tryptophanyl tRNA synthetase/WRS in paraffin-embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
References
ab109213 has not yet been referenced specifically in any publications.
