Product nameAnti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423]
See all Tryptophanyl tRNA synthetase/WRS primary antibodies
DescriptionRabbit monoclonal [EPR3423] to Tryptophanyl tRNA synthetase/WRS
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Tryptophanyl tRNA synthetase/WRS aa 50-150. The exact sequence is proprietary.
- HeLa, JAR, 293T and L6 cell lysates; Human breast tissue
Previously labelled as Tryptophanyl tRNA synthetase.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 53 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionIsoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 WHEP-TRS domain.
modificationsProteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
- Information by UniProt
- Gamma 2 antibody
- GAMMA-2 antibody
- Gamma2 antibody
All lanes : Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : 293T cell lysate
Lane 4 : L6 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDa
ab109213, at 1/500 dilution, staining Tryptophanyl tRNA synthetase/WRS in paraffin-embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab109213 has not yet been referenced specifically in any publications.