RecombinantRabMAb

Recombinant Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)

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Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
  • Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3423] to Tryptophanyl tRNA synthetase/WRS
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423]
    See all Tryptophanyl tRNA synthetase/WRS primary antibodies

  • Description

    Rabbit monoclonal [EPR3423] to Tryptophanyl tRNA synthetase/WRS

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, JAR, 293T and L6 cell lysates; Human breast tissue

  • General notes

     

     

    Previously labelled as Tryptophanyl tRNA synthetase. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function

      Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.

    • Sequence similarities

      Belongs to the class-I aminoacyl-tRNA synthetase family.
      Contains 1 WHEP-TRS domain.

    • Post-translational
      modifications

      Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Gamma 2 antibody
      • GAMMA-2 antibody
      • Gamma2 antibody
      • hWRS antibody
      • IFI 53 antibody
      • IFI53 antibody
      • IFP 53 antibody
      • IFP53 antibody
      • Interferon induced protein 53 antibody
      • Interferon-induced protein 53 antibody
      • SYWC_HUMAN antibody
      • T2-TrpRS antibody
      • TrpRS antibody
      • Tryptophan tRNA ligase, cytoplasmic antibody
      • Tryptophan tRNA ligase 1 cytoplasmic antibody
      • Tryptophan tRNA ligase antibody
      • Tryptophan--tRNA ligase antibody
      • Tryptophanyl tRNA synthetase antibody
      • Tryptophanyl tRNA synthetase cytoplasmic antibody
      • WARS antibody
      • WARS protein antibody
      • WRS antibody
      see all

    Images

    • Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
      Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
      All lanes : Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : L6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 53 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)

      ab109213, at 1/500 dilution, staining Tryptophanyl tRNA synthetase/WRS in paraffin-embedded Human breast tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
      Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)

    References (0)

    Publishing research using ab109213? Please let us know so that we can cite the reference in this datasheet.

    ab109213 has not yet been referenced specifically in any publications.

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