Key features and details
- Rabbit polyclonal to TSC-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TSC-1 antibody
See all TSC-1 primary antibodies
DescriptionRabbit polyclonal to TSC-1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human lung cancer and mammary cancer tissues. WB: Colo320, A549 and HT1080 whole cell lysates.
Previously labelled as Eotaxin 3.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab217328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
The detection limit for Eotaxin 3 is approximately 0.25 ng/lane under reducing conditions.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionChemotactic for eosinophils and basophils. Binds to CCR3.
Tissue specificityUbiquitously expressed at low levels in various tissues including heart and ovary.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
- Information by UniProt
- C-C motif chemokine 26 antibody
- CC chemokine IMAC antibody
- CCL 26 antibody
All lanes : Anti-TSC-1 antibody (ab217328) at 0.5 µg/ml
Lane 1 : Colo320 whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : HT1080 whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 11 kDa
Immunohistochemical analysis of paraffin-embedded human mammary cancer tissue labeling TSC-1 with ab217328 at 1 µg/mL.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling TSC-1 with ab217328 at 1 µg/mL.
ab217328 has been referenced in 1 publication.
- Jiang Y et al. TIPE2 governs macrophage polarization via negative regulation of mTORC1. Mol Med Rep 17:952-960 (2018). PubMed: 29115630