Key features and details
- Mouse polyclonal to TSEN34
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TSEN34 antibody
See all TSEN34 primary antibodies
DescriptionMouse polyclonal to TSEN34
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length human TSEN34 protein (AAH00944.1, 390 a.a)
- TSEN34 transfected 293T cell lysate.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituents: 0.2% Monobasic dihydrogen potassium phosphate, 0.6% Dibasic monohydrogen sodium phosphate, 8% Sodium chloride, 0.2% Potassium chloride, 91% dH2O
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).|
FunctionConstitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.
Involvement in diseasePontocerebellar hypoplasia 2C
Sequence similaritiesBelongs to the tRNA-intron endonuclease family.
Cellular localizationNucleus. Nucleus, nucleolus. May be transiently localized in the nucleolus.
- Information by UniProt
- HsSen34 antibody
- LENG 5 antibody
- LENG5 antibody
All lanes : Anti-TSEN34 antibody (ab68868) at 1/500 dilution
Lane 1 : TSEN34 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
ab68868 has been referenced in 1 publication.
- Breuss MW et al. Autosomal-Recessive Mutations in the tRNA Splicing Endonuclease Subunit TSEN15 Cause Pontocerebellar Hypoplasia and Progressive Microcephaly. Am J Hum Genet 99:228-35 (2016). PubMed: 27392077