Key features and details
- Mouse monoclonal [OTI6B3] to TSEN34
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-TSEN34 antibody [OTI6B3]
See all TSEN34 primary antibodies
DescriptionMouse monoclonal [OTI6B3] to TSEN34
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human TSEN34 aa 1-310. Produced in E.coli (NP_076980).
MLVVEVANGRSLVWGAEAVQALRERLGVGGRTVGALPRGPRQNSRLGLPL LLMPEEARLLAEIGAVTLVSAPRPDSRHHSLALTSFKRQQEESFQEQSAL AAEARETRRQELLEKITEGQAAKKQKLEQASGASSSQEAGSSQAAKEDET SDGQASGEQEEAGPSSSQAGPSNGVAPLPRSALLVQLATARPRPVKARPL DWRVQSKDWPHAGRPAHELRYSIYRDLWERGFFLSAAGKFGGDFLVYPGD PLRFHAHYIAQCWAPEDTIPLQDLVAAGRLGTSVRKTLLLCSPQPDGKVV YTSLQWASLQ
Database link: Q9BSV6
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY TSEN34 cDNA. IHC-P: Human colon adenocarcinoma, kidney carcinoma, lung carcinoma and endometrium tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 34 kDa.|
Perform heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH 8.5) at 120°C for 3 minutes.
FunctionConstitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.
Involvement in diseasePontocerebellar hypoplasia 2C
Sequence similaritiesBelongs to the tRNA-intron endonuclease family.
Cellular localizationNucleus. Nucleus, nucleolus. May be transiently localized in the nucleolus.
- Information by UniProt
- HsSen34 antibody
- LENG 5 antibody
- LENG5 antibody
All lanes : Anti-TSEN34 antibody [OTI6B3] (ab236423) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY TSEN34 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 34 kDa
Paraffin-embedded human colon adenocarcinoma tissue stained for TSEN34 using ab236423 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney carcinoma tissue stained for TSEN34 using ab236423 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human lung carcinoma tissue stained for TSEN34 using ab236423 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human endometrium tissue stained for TSEN34 using ab236423 at 1/150 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236423 has not yet been referenced specifically in any publications.