Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10062] to TSEN54
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-TSEN54 antibody [EPR10062]
See all TSEN54 primary antibodies
DescriptionRabbit monoclonal [EPR10062] to TSEN54
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TSEN54 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q7Z6J9
- MCF7, 293T and Y79 cell lysates; Human pancreas tissue; MCF7 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab178696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 59 kDa.|
|ICC/IF||1/100 - 1/250.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionNon-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3' end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3' end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.
Involvement in diseaseDefects in TSEN54 are the cause of pontocerebellar hypoplasia type 4 (PCH4) [MIM:225753]. Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. PCH4 is characterized by severe course and early lethality.
Defects in TSEN54 are the cause of pontocerebellar hypoplasia type 2A (PCH2A) [MIM:277470]. Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. PCH type 2 is characterized by progressive microcephaly from birth combined with extrapyramidal dyskinesia and chorea, epilepsy, and normal spinal cord findings.
Sequence similaritiesBelongs to the SEN54 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Nucleus > nucleolus. May be transiently localized in the nucleolus.
- Information by UniProt
- HsSen54 antibody
- PCH2A antibody
- PCH4 antibody
All lanes : Anti-TSEN54 antibody [EPR10062] (ab178696) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 59 kDa
Immunofluorescent analysis of MCF7 cells labeling TSEN54 with ab178696 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TSEN54 with ab178696 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab178696 has been referenced in 1 publication.
- Breuss MW et al. Autosomal-Recessive Mutations in the tRNA Splicing Endonuclease Subunit TSEN15 Cause Pontocerebellar Hypoplasia and Progressive Microcephaly. Am J Hum Genet 99:228-35 (2016). PubMed: 27392077